The role of the laterodorsal tegmental nucleus in methamphetamine conditioned place preference and locomotor activity

Lauren K. Dobbs, Christopher L. Cunningham

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Methamphetamine (METH) indirectly stimulates the laterodorsal tegmental nucleus (LDT) acetylcholine (ACh) neurons to increase ACh within the ventral tegmental area (VTA). LDT ACh inhibition attenuates METH and saline locomotor activity. The aim of these experiments was to determine whether LDT ACh contributes to METH conditioned place preference (CPP). C57BL/6. J mice received a bilateral electrolytic or sham lesion of the LDT. After recovery, mice received alternating pairings of METH (0.5. mg/kg) and saline with distinct tactile floor cues over 8 days. During preference tests, mice were given access to both floor types and time spent on each was recorded. Mice were tested again after exposure to both extinction and reconditioning trials. Brains were then processed for choline acetyltransferase immunohistochemistry to label LDT ACh neurons. Lesioned mice had significantly fewer LDT ACh neurons and showed increased saline and METH locomotor activity during the first conditioning trial compared to sham mice. Locomotor activity (saline and METH) was negatively correlated with the number of LDT ACh neurons. Lesioned and sham mice showed similar METH CPP following conditioning, extinction and reconditioning trials. LDT ACh neurons are not necessary for METH reward as indexed by CPP, but may be important for basal and METH-induced locomotor activity.

Original languageEnglish (US)
Pages (from-to)198-202
Number of pages5
JournalBehavioural Brain Research
Volume265
DOIs
StatePublished - May 15 2014

Keywords

  • Acetylcholine
  • Electrolytic lesion
  • Inbred mice (C57BL/6)
  • Locomotor activity
  • Methamphetamine
  • Reward

ASJC Scopus subject areas

  • Behavioral Neuroscience

Fingerprint Dive into the research topics of 'The role of the laterodorsal tegmental nucleus in methamphetamine conditioned place preference and locomotor activity'. Together they form a unique fingerprint.

  • Cite this