TY - JOUR
T1 - The role of selective transport in neuronal protein sorting
AU - Burack, Michelle A.
AU - Silverman, Michael A.
AU - Banker, Gary
N1 - Funding Information:
We thank J. Muyskens for the preparation of neuronal cultures; N. Glicksman for assistance with motion analysis; and A. M. Craig, C. Enns, A. Futerman, S. Green, M. Jareb, S. Kaech, and B. Sampo for their comments on the manuscript. This work was supported by National Institutes of Health grant NS17112.
PY - 2000
Y1 - 2000
N2 - To assess whether selective microtubule-based vesicle transport underlies the polarized distribution of neuronal proteins, we expressed green fluorescent protein- (GFP-) tagged chimeras of representative axonal and dendritic membrane proteins in cultured hippocampal neurons and visualized the transport of carrier vesicles containing these proteins in living cells. Vesicles containing a dendritic protein, transferrin receptor (TfR), were preferentially transported into dendrites and excluded from axons. In contrast, vesicles containing the axonal protein NgCAM (neuron-glia cell adhesion molecule) were transported into both dendrites and axons. These data demonstrate that neurons utilize two distinct mechanisms for the targeting of polarized membrane proteins, one (for dendritic proteins) based on selective transport, the other (for axonal proteins) based on a selectivity 'filter' that occurs downstream of transport.
AB - To assess whether selective microtubule-based vesicle transport underlies the polarized distribution of neuronal proteins, we expressed green fluorescent protein- (GFP-) tagged chimeras of representative axonal and dendritic membrane proteins in cultured hippocampal neurons and visualized the transport of carrier vesicles containing these proteins in living cells. Vesicles containing a dendritic protein, transferrin receptor (TfR), were preferentially transported into dendrites and excluded from axons. In contrast, vesicles containing the axonal protein NgCAM (neuron-glia cell adhesion molecule) were transported into both dendrites and axons. These data demonstrate that neurons utilize two distinct mechanisms for the targeting of polarized membrane proteins, one (for dendritic proteins) based on selective transport, the other (for axonal proteins) based on a selectivity 'filter' that occurs downstream of transport.
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U2 - 10.1016/S0896-6273(00)81178-2
DO - 10.1016/S0896-6273(00)81178-2
M3 - Article
C2 - 10839364
AN - SCOPUS:0033697715
SN - 0896-6273
VL - 26
SP - 465
EP - 472
JO - Neuron
JF - Neuron
IS - 2
ER -