The role of protein kinase C on amylase secretion from rat parotid gland

Hiromi Shimomura, Akane Terada, Yoshiaki Hashimoto, Thomas R. Soderling

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    Abstract

    The activities of Ca2+·phospholipid-dependent protein kinase(protein kinase C) in rat salivary gland were assayed using synthetic peptide syntide-2(Pro-Leu-Ala-Arg-Thr-Leu-Ser-Val-Ala-Gly-Leu-Pro-Gly-Lys-Lys) as substrate. Levels of the protein kinase C were less than 0.05units/g in the parotid and submandibular glands. The protein kinase C inhibitor, H-7, inhibited amylase secretion from rat parotid gland stimulated by PMA or the combination of phosphatidylserine and 1,2-diolein. The results supported the hypothesis of the secretory mechanism that protein kinase C mediates amylase secretion in rat parotid glands.

    Original languageEnglish (US)
    Pages (from-to)1309-1314
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume150
    Issue number3
    DOIs
    StatePublished - Feb 15 1988

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    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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