The role of protein kinase C on amylase secretion from rat parotid gland

Hiromi Shimomura, Akane Terada, Yoshiaki Hashimoto, Thomas Soderling

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The activities of Ca2+·phospholipid-dependent protein kinase(protein kinase C) in rat salivary gland were assayed using synthetic peptide syntide-2(Pro-Leu-Ala-Arg-Thr-Leu-Ser-Val-Ala-Gly-Leu-Pro-Gly-Lys-Lys) as substrate. Levels of the protein kinase C were less than 0.05units/g in the parotid and submandibular glands. The protein kinase C inhibitor, H-7, inhibited amylase secretion from rat parotid gland stimulated by PMA or the combination of phosphatidylserine and 1,2-diolein. The results supported the hypothesis of the secretory mechanism that protein kinase C mediates amylase secretion in rat parotid glands.

Original languageEnglish (US)
Pages (from-to)1309-1314
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume150
Issue number3
DOIs
StatePublished - Feb 15 1988
Externally publishedYes

Fingerprint

syntide-2
Parotid Gland
Amylases
Protein Kinase C
Rats
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Protein C Inhibitor
Submandibular Gland
Phosphatidylserines
Protein Kinase Inhibitors
Salivary Glands
Protein Kinases
Phospholipids
Peptides
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The role of protein kinase C on amylase secretion from rat parotid gland. / Shimomura, Hiromi; Terada, Akane; Hashimoto, Yoshiaki; Soderling, Thomas.

In: Biochemical and Biophysical Research Communications, Vol. 150, No. 3, 15.02.1988, p. 1309-1314.

Research output: Contribution to journalArticle

Shimomura, Hiromi ; Terada, Akane ; Hashimoto, Yoshiaki ; Soderling, Thomas. / The role of protein kinase C on amylase secretion from rat parotid gland. In: Biochemical and Biophysical Research Communications. 1988 ; Vol. 150, No. 3. pp. 1309-1314.
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