The role of protein kinase C and calcium in induction of human polymorphonuclear leukocyte IL-1β gene expression by GM-CSP

Marilyn C. Fernandez, Phillip Marucha, Isolde G. Rojas, John D. Walters

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

At infection sites, synthesis of interleukin (IL-)1β by polymorphonuclear leukocytes (PMNs) facilitates the recruitment of inflammatory cells and enhances the inflammatory response. We investigated the role of protein kinase C (PKC) and Ca2+ in the induction of PMN IL-1β gene expression by GM-CSF. The PKC inhibitors chelerythrine and H7 blocked induction of IL-1β mRNA expression in human PMNs. HA1004, an H7 analogue with little activity towards PKC, had no inhibitory effect. Similarly, H7 blocked IL-1β transcription in nuclear run-on analysis, while HA1004 had little effect. The intracellular Ca2+ chelator BAPTA/AM inhibited induction of IL-1β mRNA accumulation and transcription by GM-CSF. At concentrations similar to those used to inhibit IL-1β gene expression, H7, chelerythrine, and BAPTA all inhibited substrate phosphorylation by PKC isolated from PMN lysates. Thus, PKC and Ca2+ are potential targets for modulating an important PMN immunoregulatory function. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)445-449
Number of pages5
JournalCytokine
Volume12
Issue number5
DOIs
StatePublished - May 2000
Externally publishedYes

Fingerprint

Interleukin-1
Gene expression
Protein Kinase C
Neutrophils
Calcium
Gene Expression
Transcription
Granulocyte-Macrophage Colony-Stimulating Factor
Messenger RNA
Phosphorylation
Protein C Inhibitor
Protein Kinase Inhibitors
Chelating Agents
Substrates
Infection

Keywords

  • Gene expression
  • Granulocytemacrophage colony-stimulating factor
  • Interleukin 1
  • Neutrophil

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

The role of protein kinase C and calcium in induction of human polymorphonuclear leukocyte IL-1β gene expression by GM-CSP. / Fernandez, Marilyn C.; Marucha, Phillip; Rojas, Isolde G.; Walters, John D.

In: Cytokine, Vol. 12, No. 5, 05.2000, p. 445-449.

Research output: Contribution to journalArticle

Fernandez, Marilyn C. ; Marucha, Phillip ; Rojas, Isolde G. ; Walters, John D. / The role of protein kinase C and calcium in induction of human polymorphonuclear leukocyte IL-1β gene expression by GM-CSP. In: Cytokine. 2000 ; Vol. 12, No. 5. pp. 445-449.
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