The role of nitric oxide and NMDA receptors in the development of motor neuron dendrites

Fiona M. Inglis, Fran Furia, Katharine Zuckerman, Stephen M. Strittmatter, Robert G. Kalb

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Nitric oxide (NO) has been implicated in the establishment of precise synaptic connectivity throughout the neuroaxis in several species. To determine the contribution of NO to NMDA receptor-dependent dendritic growth in motor neurons, we administered the NMDA antagonist MK-801 to wild-type mice and neuronal nitric oxide synthase (nNOS) knock-out mice between postnatal days 7 and 14. Compared to saline-treated wild-type animals the number of dendritic bifurcations was significantly reduced in nNOS knock-out animals and MK-801-treated wildtype animals. There was no significant difference in dendritic bifurcation between MK-801-treated wild-type, MK- 801-treated nNOS knock-out, and saline-treated nNOS knock-out animals, suggesting that nNOS knock-out and NMDA receptor block had similar effects. The path of the longest dendrite and the number of primary dendrites was the same in all treatment groups, indicating an effect specific to bifurcation. Sholl analysis revealed that differences in bifurcation numbers occurred between 160 and 320 μm from the cell body, the distance at which second, third, and fourth order dendrites are most prevalent. Dendrite order analyses confirmed a significant reduction in numbers, but not lengths, of third and fourth order dendrites in nNOS knock-out and drug-treatment groups. Finally, immunohistochemical examination of the developing spinal cord indicated that NMDA receptors and nNOS are colocalized within interneurons surrounding the motor neuron pool. These results support the view that at least part of NMDA receptor-dependent arborization of motor neuron dendrites is mediated by the local production of NO within the developing spinal cord.

Original languageEnglish (US)
Pages (from-to)10493-10501
Number of pages9
JournalJournal of Neuroscience
Volume18
Issue number24
StatePublished - Dec 15 1998
Externally publishedYes

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Nitric Oxide Synthase Type I
Motor Neurons
Dendrites
N-Methyl-D-Aspartate Receptors
Dizocilpine Maleate
Nitric Oxide
Spinal Cord
Wild Animals
Interneurons
N-Methylaspartate
Soluble Guanylyl Cyclase
Knockout Mice
Growth
Pharmaceutical Preparations

Keywords

  • Dendrite
  • Development
  • Motor neuron
  • Nitric oxide
  • Nitric oxide synthase
  • NMDA receptor
  • Spinal cord
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Inglis, F. M., Furia, F., Zuckerman, K., Strittmatter, S. M., & Kalb, R. G. (1998). The role of nitric oxide and NMDA receptors in the development of motor neuron dendrites. Journal of Neuroscience, 18(24), 10493-10501.

The role of nitric oxide and NMDA receptors in the development of motor neuron dendrites. / Inglis, Fiona M.; Furia, Fran; Zuckerman, Katharine; Strittmatter, Stephen M.; Kalb, Robert G.

In: Journal of Neuroscience, Vol. 18, No. 24, 15.12.1998, p. 10493-10501.

Research output: Contribution to journalArticle

Inglis, FM, Furia, F, Zuckerman, K, Strittmatter, SM & Kalb, RG 1998, 'The role of nitric oxide and NMDA receptors in the development of motor neuron dendrites', Journal of Neuroscience, vol. 18, no. 24, pp. 10493-10501.
Inglis, Fiona M. ; Furia, Fran ; Zuckerman, Katharine ; Strittmatter, Stephen M. ; Kalb, Robert G. / The role of nitric oxide and NMDA receptors in the development of motor neuron dendrites. In: Journal of Neuroscience. 1998 ; Vol. 18, No. 24. pp. 10493-10501.
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