The role of macrophages in thymocyte mitogenesis

Gordon Mills, V. Monticone, V. Paetkau

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The thymic lymphocytes of CBA/J mice respond to the mitogen Concanavalin A (Con A) only in the presence of adherent cells of the monocyte macrophage series. Depletion of adherent cells abrogates the response, and macrophage rich populations of cells restore it. The need for macrophages and mitogen is completely provided by irradiated splenic macrophages which have been exposed to Con A and washed free of the soluble mitogen. The mitogen macrophage effect in this case is apparently not due to soluble factors. Even more striking than the effect of macrophages on fresh cultures of thymic lymphocytes is their ability to restimulate quiescent cells 72 hr after their first stimulation with Con A. The quiescent cells respond immediately and quantitatively to Con A in the presence of fresh macrophages. This stimulation, like that of fresh thymocytes, is also controlled by a lymphokine (''costimulator'') produced by mixing macrophages, mitogen, and T lymphocytes. These data suggest a model in which two signals are required for mitogenesis. First, the interaction of macrophage, T cell, and mitogen elicits a soluble costimulator, which is itself not mitogenic. Secondly, in the presence of costimulator, the mitogen (either soluble, or, more efficiently, bound to macrophages) induces a proliferative response in the T cell.

Original languageEnglish (US)
Pages (from-to)1325-1330
Number of pages6
JournalJournal of Immunology
Volume117
Issue number4
StatePublished - Dec 1 1976
Externally publishedYes

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Thymocytes
Macrophages
Mitogens
Concanavalin A
T-Lymphocytes
Lymphocytes
Inbred CBA Mouse
Lymphokines
Monocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Mills, G., Monticone, V., & Paetkau, V. (1976). The role of macrophages in thymocyte mitogenesis. Journal of Immunology, 117(4), 1325-1330.

The role of macrophages in thymocyte mitogenesis. / Mills, Gordon; Monticone, V.; Paetkau, V.

In: Journal of Immunology, Vol. 117, No. 4, 01.12.1976, p. 1325-1330.

Research output: Contribution to journalArticle

Mills, G, Monticone, V & Paetkau, V 1976, 'The role of macrophages in thymocyte mitogenesis', Journal of Immunology, vol. 117, no. 4, pp. 1325-1330.
Mills G, Monticone V, Paetkau V. The role of macrophages in thymocyte mitogenesis. Journal of Immunology. 1976 Dec 1;117(4):1325-1330.
Mills, Gordon ; Monticone, V. ; Paetkau, V. / The role of macrophages in thymocyte mitogenesis. In: Journal of Immunology. 1976 ; Vol. 117, No. 4. pp. 1325-1330.
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