The role of IL-2 interaction with p75 and p55 receptor molecules in the stimulation of cell proliferation

G. R. Perkins, C. L. Flemming, David Kabat, M. K L Collins

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This report addresses the role of individual IL-2 binding proteins of the IL-2 receptor in the stimulation of cell proliferation by IL-2. Murine IL-3-dependent cell lines were established which expressed the human p75 IL-2-binding protein, in the absence or presence of the human p55 IL-2-binding protein. Whereas p75 expression was sufficient to confer response to an intermediate (half-maximal stimulation at 100 pM) concentration of IL-2, additional expression of p55 increased the sensitivity of the cells to half-maximal stimulation at 10 pM IL-2. A mutant IL-2 molecule, Lys-20 IL-2 which is known to be defective of p75 interaction, was unable to stimulate cells expressing only p75: p55 co-expression could restore its activity. Under conditions of low p75 expression, Lys-20 IL-2 could act as an antagonist of wild-type IL-2 action. These data support a role for p55 in the enhancement of responsiveness to IL-2.

Original languageEnglish (US)
Pages (from-to)541-549
Number of pages9
JournalInternational Immunology
Volume5
Issue number5
StatePublished - 1993

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Interleukin-2
Cell Proliferation
Carrier Proteins
Interleukin-2 Receptors
Interleukin-3
Cell Line

Keywords

  • Antagonist
  • Cytokine

ASJC Scopus subject areas

  • Immunology

Cite this

Perkins, G. R., Flemming, C. L., Kabat, D., & Collins, M. K. L. (1993). The role of IL-2 interaction with p75 and p55 receptor molecules in the stimulation of cell proliferation. International Immunology, 5(5), 541-549.

The role of IL-2 interaction with p75 and p55 receptor molecules in the stimulation of cell proliferation. / Perkins, G. R.; Flemming, C. L.; Kabat, David; Collins, M. K L.

In: International Immunology, Vol. 5, No. 5, 1993, p. 541-549.

Research output: Contribution to journalArticle

Perkins, GR, Flemming, CL, Kabat, D & Collins, MKL 1993, 'The role of IL-2 interaction with p75 and p55 receptor molecules in the stimulation of cell proliferation', International Immunology, vol. 5, no. 5, pp. 541-549.
Perkins, G. R. ; Flemming, C. L. ; Kabat, David ; Collins, M. K L. / The role of IL-2 interaction with p75 and p55 receptor molecules in the stimulation of cell proliferation. In: International Immunology. 1993 ; Vol. 5, No. 5. pp. 541-549.
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