The role of BiP in endoplasmic reticulum-associated degradation of major histocompatibility complex class I heavy chain induced by cytomegalovirus proteins

Nagendra R. Hegde, Mathieu S. Chevalier, Todd W. Wisner, Michael C. Denton, Kathy Shire, Lori Frappier, David Johnson

Research output: Contribution to journalArticle

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Abstract

Human cytomegalovirus (HCMV1) US11 and US2 proteins cause rapid degradation of major histocompatibility complex (MHC) molecules, apparently by ligating cellular endoplasmic reticulum (ER)-associated degradation machinery. Here, we show that US11 and US2 bind the ER chaperone BiP. Four related HCMV proteins, US3, US7, US9, and US10, which do not promote degradation of MHC proteins, did not bind BiP. Silencing BiP reduced US11- and US2-mediated degradation of MHC class I heavy chain (HC) without altering the synthesis or translocation of HC into the ER or the stability of HC in the absence of US11 or US2. Induction of the unfolded protein response (UPR) did not affect US11-mediated HC degradation and could not explain the stabilization of HC when BiP was silenced. Unlike in yeast, BiP did not act by maintaining substrates in a retrotranslocation- competent form. Our studies go beyond previous observations in mammalian cells correlating BiP release with degradation, demonstrating that BiP is functionally required for US2- and US11-mediated HC degradation. Further, US2 and US11 bound BiP even when HC was absent and degradation of US2 depended on HC. These data were consistent with a model in which US2 and US11 bridge HC onto BiP promoting interactions with other ER-associated degradation proteins.

Original languageEnglish (US)
Pages (from-to)20910-20919
Number of pages10
JournalJournal of Biological Chemistry
Volume281
Issue number30
DOIs
StatePublished - Jul 28 2006

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Endoplasmic Reticulum-Associated Degradation
Major Histocompatibility Complex
Cytomegalovirus
Degradation
Endoplasmic Reticulum
Unfolded Protein Response
Proteins
Yeasts
Yeast
Machinery
Stabilization
Cells

ASJC Scopus subject areas

  • Biochemistry

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The role of BiP in endoplasmic reticulum-associated degradation of major histocompatibility complex class I heavy chain induced by cytomegalovirus proteins. / Hegde, Nagendra R.; Chevalier, Mathieu S.; Wisner, Todd W.; Denton, Michael C.; Shire, Kathy; Frappier, Lori; Johnson, David.

In: Journal of Biological Chemistry, Vol. 281, No. 30, 28.07.2006, p. 20910-20919.

Research output: Contribution to journalArticle

Hegde, Nagendra R. ; Chevalier, Mathieu S. ; Wisner, Todd W. ; Denton, Michael C. ; Shire, Kathy ; Frappier, Lori ; Johnson, David. / The role of BiP in endoplasmic reticulum-associated degradation of major histocompatibility complex class I heavy chain induced by cytomegalovirus proteins. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 30. pp. 20910-20919.
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