The ribosome-Sec61 translocon complex forms a cytosolically restricted environment for early polytopic membrane protein folding

Melissa A. Patterson, Anannya Bandyopadhyay, Prasanna K. Devaraneni, Josha Woodward, Leeann Rooney, Zhongying Yang, William R. Skach

    Research output: Contribution to journalArticlepeer-review

    5 Scopus citations

    Abstract

    Background: Mechanisms that guide membrane protein folding in the endoplasmic reticulum membrane remain unresolved. Results: During aquaporin-4 synthesis, extracellular peptides loops enter the endoplasmic reticulum lumen sequentially, whereas delivery of cytosolic loops is actively delayed. Conclusion: The assembled ribosome translocon complex (RTC) shields large regions of the protein from the cytosol throughout synthesis. Significance: Early membrane protein folding occurs in a proteinaceous environment provided by the RTC.

    Original languageEnglish (US)
    Pages (from-to)28944-28952
    Number of pages9
    JournalJournal of Biological Chemistry
    Volume290
    Issue number48
    DOIs
    StatePublished - Nov 27 2015

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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