TY - JOUR
T1 - The ribosome-Sec61 translocon complex forms a cytosolically restricted environment for early polytopic membrane protein folding
AU - Patterson, Melissa A.
AU - Bandyopadhyay, Anannya
AU - Devaraneni, Prasanna K.
AU - Woodward, Josha
AU - Rooney, Leeann
AU - Yang, Zhongying
AU - Skach, William R.
N1 - Publisher Copyright:
© 2015 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A.
PY - 2015/11/27
Y1 - 2015/11/27
N2 - Background: Mechanisms that guide membrane protein folding in the endoplasmic reticulum membrane remain unresolved. Results: During aquaporin-4 synthesis, extracellular peptides loops enter the endoplasmic reticulum lumen sequentially, whereas delivery of cytosolic loops is actively delayed. Conclusion: The assembled ribosome translocon complex (RTC) shields large regions of the protein from the cytosol throughout synthesis. Significance: Early membrane protein folding occurs in a proteinaceous environment provided by the RTC.
AB - Background: Mechanisms that guide membrane protein folding in the endoplasmic reticulum membrane remain unresolved. Results: During aquaporin-4 synthesis, extracellular peptides loops enter the endoplasmic reticulum lumen sequentially, whereas delivery of cytosolic loops is actively delayed. Conclusion: The assembled ribosome translocon complex (RTC) shields large regions of the protein from the cytosol throughout synthesis. Significance: Early membrane protein folding occurs in a proteinaceous environment provided by the RTC.
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U2 - 10.1074/jbc.M115.672261
DO - 10.1074/jbc.M115.672261
M3 - Article
C2 - 26254469
AN - SCOPUS:84948452827
SN - 0021-9258
VL - 290
SP - 28944
EP - 28952
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 48
ER -