The rhesus macaque β1-adrenergic receptor gene: Structure of the gene and comparison of the flanking sequences with the rat β1-adrenergic receptor gene

Robert Searles, Valerie J. Nipper, Curtis Machida

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have cloned the gene for the rhesus macaque β1-adrenergic receptor. In addition to the protein coding block, we have sequenced its 5' (1424 bp) and 3' (1534 bp) flanking regions and aligned them with comparable sections of the rat β1-adrenergic receptor gene. The rhesus macaque gene contains a 1440 bp open reading frame which codes for a deduced protein of 480 amino acids that is 95% and 89% similar to the human and rat β1-adrenergic receptors, respectively. The rhesus macaque β1-adrenergic receptor contains conserved sites for potential Nlinked glycosylation and cAMP-dependent protein kinase phosphorylation identified within the human and rat receptors, but differs in the structure and length of the third cytoplasmic loop. The 400 bases of 5' flanking sequence proximal to the protein coding block are highly conserved (84% similarity) between the rat and rhesus macaque genes. The entire 3' flanking sequence, which extends beyond two potential polyadenylation sites at 1050 and 1337 bp relative to the translation termination codon, is also highly conserved between the two species. Comparison of the flanking sequences of the two species reveals conserved regulatory sequences which may be important for β1-adrenergic receptor expression and transcriptional modulation.

Original languageEnglish (US)
Pages (from-to)231-241
Number of pages11
JournalMitochondrial DNA
Volume4
Issue number4
DOIs
StatePublished - 1994

Fingerprint

Macaca mulatta
Adrenergic Receptors
Rats
Genes
3' Flanking Region
Glycosylation
Polyadenylation
Phosphorylation
Proteins
Terminator Codon
Conserved Sequence
5' Flanking Region
Cyclic AMP-Dependent Protein Kinases
Open Reading Frames
Modulation
Amino Acids

Keywords

  • Flanking sequences
  • Gene structure
  • Transcriptional regulation

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry
  • Endocrinology

Cite this

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abstract = "We have cloned the gene for the rhesus macaque β1-adrenergic receptor. In addition to the protein coding block, we have sequenced its 5' (1424 bp) and 3' (1534 bp) flanking regions and aligned them with comparable sections of the rat β1-adrenergic receptor gene. The rhesus macaque gene contains a 1440 bp open reading frame which codes for a deduced protein of 480 amino acids that is 95{\%} and 89{\%} similar to the human and rat β1-adrenergic receptors, respectively. The rhesus macaque β1-adrenergic receptor contains conserved sites for potential Nlinked glycosylation and cAMP-dependent protein kinase phosphorylation identified within the human and rat receptors, but differs in the structure and length of the third cytoplasmic loop. The 400 bases of 5' flanking sequence proximal to the protein coding block are highly conserved (84{\%} similarity) between the rat and rhesus macaque genes. The entire 3' flanking sequence, which extends beyond two potential polyadenylation sites at 1050 and 1337 bp relative to the translation termination codon, is also highly conserved between the two species. Comparison of the flanking sequences of the two species reveals conserved regulatory sequences which may be important for β1-adrenergic receptor expression and transcriptional modulation.",
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