The reverse cholesterol transport system as a potential mediator of luteolysis in the primate corpus luteum

Randy L. Bogan, Jon Hennebold

    Research output: Contribution to journalArticle

    27 Citations (Scopus)

    Abstract

    The cessation of progesterone (P4) production (i.e. functional regression), arguably the key event in luteolysis of the primate corpus luteum (CL), is poorly understood. Previously, we found that genes encoding proteins involved in cholesterol uptake decreased, while those involved in cholesterol efflux (reverse cholesterol transport, RCT) increased in expression during spontaneous functional regression of the rhesus macaque CL, thereby potentially depleting the cholesterol reserves needed for steroidogenesis. Therefore, a comprehensive analysis of the components necessary for RCT was performed. RCT components were expressed (mRNA and/or protein) in the macaque CL including cholesterol sensors (liver X receptors a or NR1H3; and b or NR1H2), efflux proteins (ATP-binding cassette subfamilies A1 (ABCA1) and G1), acceptors (apolipoproteins A1 or APOA1; and E or APOE), and plasma proteins facilitating high-density lipoprotein formation (lecithin:cholesterol acyltransferase or LCAT; phospholipid transfer protein or PLTP). ABCA1, APOE, PLTP, and NR1H3 increased, while lipoprotein receptors decreased, in expression (mRNA and/or protein) through the period of functional regression. The expression of APOA1 and APOE, as well as NR1H3, was greatest in the CL and tissues involved in regulating cholesterol homeostasis. Immunolocalization studies revealed that RCT proteins and lipoprotein receptors were expressed in large luteal cells, which possess intracellular cholesterol reserves during periods of P4 synthesis. Lipid staining revealed changes in luteal cholesterol ester/lipid distribution that occurred following functional regression. These results indicate that decreased cholesterol uptake and increased RCT may be critical for the initiation of primate luteolysis by limiting intracellular cholesterol pools required for steroidogenesis.

    Original languageEnglish (US)
    Pages (from-to)163-176
    Number of pages14
    JournalReproduction
    Volume139
    Issue number1
    DOIs
    StatePublished - Jan 2010

    Fingerprint

    Luteolysis
    Corpus Luteum
    Primates
    Cholesterol
    Lipoprotein Receptors
    Adenosine Triphosphate
    Period Circadian Proteins
    Phospholipid Transfer Proteins
    Phosphatidylcholine-Sterol O-Acyltransferase
    Lipids
    Luteal Cells
    Messenger RNA
    Apolipoproteins
    Cholesterol Esters
    Macaca
    HDL Lipoproteins
    Macaca mulatta
    Protein Binding
    Progesterone
    Blood Proteins

    ASJC Scopus subject areas

    • Obstetrics and Gynecology
    • Reproductive Medicine
    • Embryology
    • Endocrinology
    • Cell Biology

    Cite this

    The reverse cholesterol transport system as a potential mediator of luteolysis in the primate corpus luteum. / Bogan, Randy L.; Hennebold, Jon.

    In: Reproduction, Vol. 139, No. 1, 01.2010, p. 163-176.

    Research output: Contribution to journalArticle

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