The regulatory role of Interleukin 2-responsive T lymphocytes on human marrow granulopoiesis

Z. Estrov, C. Roifman, Gordon Mills, T. Grunberger, E. W. Gelfand, M. H. Freedman

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effect of recombinant interleukin 2 (IL2) on marrow CFU-C colony formation was evaluated to define the role for T lymphocytes in human marrow granulopoiesis. The colony-stimulating factor (CSA) used in our experiments was found to contain IL2. IL2 depletion from CSA resulted in a reduction in CFU-C colony proliferation. Addition of exogenous IL2 caused an increase in CFU-C colony numbers in a dose-dependent manner. This increase could be prevented by anti-Tac, a monoclonal antibody (MoAb) to the IL2 receptor. Moreover, anti-Tac in the absence of exogenous IL2 resulted in an overall decrease in colony numbers. Depletion of either adherent cells or T lymphocytes abolished the effect of IL2 and anti-Tac on colony growth. In the presence of IL2, re-addition of T lymphocytes to the T-depleted marrow or adherent cells to adherent cell-depleted marrow resulted in a significant increase in CFU-C colony numbers, whereas no significant effect was found when IL2-depleted CSA was used. Although T lymphocytes were not themselves essential for CFU-C colony growth, our studies indicate that IL2 and IL2-responsive T cells can regulate in vitro granulopoiesis.

Original languageEnglish (US)
Pages (from-to)1161-1166
Number of pages6
JournalBlood
Volume69
Issue number4
StatePublished - Jul 30 1987
Externally publishedYes

Fingerprint

T-cells
Interleukin-2
Bone Marrow
T-Lymphocytes
Colony-Stimulating Factors
Interleukin-2 Receptors
Growth
Monoclonal Antibodies

ASJC Scopus subject areas

  • Hematology

Cite this

Estrov, Z., Roifman, C., Mills, G., Grunberger, T., Gelfand, E. W., & Freedman, M. H. (1987). The regulatory role of Interleukin 2-responsive T lymphocytes on human marrow granulopoiesis. Blood, 69(4), 1161-1166.

The regulatory role of Interleukin 2-responsive T lymphocytes on human marrow granulopoiesis. / Estrov, Z.; Roifman, C.; Mills, Gordon; Grunberger, T.; Gelfand, E. W.; Freedman, M. H.

In: Blood, Vol. 69, No. 4, 30.07.1987, p. 1161-1166.

Research output: Contribution to journalArticle

Estrov, Z, Roifman, C, Mills, G, Grunberger, T, Gelfand, EW & Freedman, MH 1987, 'The regulatory role of Interleukin 2-responsive T lymphocytes on human marrow granulopoiesis', Blood, vol. 69, no. 4, pp. 1161-1166.
Estrov Z, Roifman C, Mills G, Grunberger T, Gelfand EW, Freedman MH. The regulatory role of Interleukin 2-responsive T lymphocytes on human marrow granulopoiesis. Blood. 1987 Jul 30;69(4):1161-1166.
Estrov, Z. ; Roifman, C. ; Mills, Gordon ; Grunberger, T. ; Gelfand, E. W. ; Freedman, M. H. / The regulatory role of Interleukin 2-responsive T lymphocytes on human marrow granulopoiesis. In: Blood. 1987 ; Vol. 69, No. 4. pp. 1161-1166.
@article{8f31baf82353442da90925e9701c1252,
title = "The regulatory role of Interleukin 2-responsive T lymphocytes on human marrow granulopoiesis",
abstract = "The effect of recombinant interleukin 2 (IL2) on marrow CFU-C colony formation was evaluated to define the role for T lymphocytes in human marrow granulopoiesis. The colony-stimulating factor (CSA) used in our experiments was found to contain IL2. IL2 depletion from CSA resulted in a reduction in CFU-C colony proliferation. Addition of exogenous IL2 caused an increase in CFU-C colony numbers in a dose-dependent manner. This increase could be prevented by anti-Tac, a monoclonal antibody (MoAb) to the IL2 receptor. Moreover, anti-Tac in the absence of exogenous IL2 resulted in an overall decrease in colony numbers. Depletion of either adherent cells or T lymphocytes abolished the effect of IL2 and anti-Tac on colony growth. In the presence of IL2, re-addition of T lymphocytes to the T-depleted marrow or adherent cells to adherent cell-depleted marrow resulted in a significant increase in CFU-C colony numbers, whereas no significant effect was found when IL2-depleted CSA was used. Although T lymphocytes were not themselves essential for CFU-C colony growth, our studies indicate that IL2 and IL2-responsive T cells can regulate in vitro granulopoiesis.",
author = "Z. Estrov and C. Roifman and Gordon Mills and T. Grunberger and Gelfand, {E. W.} and Freedman, {M. H.}",
year = "1987",
month = "7",
day = "30",
language = "English (US)",
volume = "69",
pages = "1161--1166",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "4",

}

TY - JOUR

T1 - The regulatory role of Interleukin 2-responsive T lymphocytes on human marrow granulopoiesis

AU - Estrov, Z.

AU - Roifman, C.

AU - Mills, Gordon

AU - Grunberger, T.

AU - Gelfand, E. W.

AU - Freedman, M. H.

PY - 1987/7/30

Y1 - 1987/7/30

N2 - The effect of recombinant interleukin 2 (IL2) on marrow CFU-C colony formation was evaluated to define the role for T lymphocytes in human marrow granulopoiesis. The colony-stimulating factor (CSA) used in our experiments was found to contain IL2. IL2 depletion from CSA resulted in a reduction in CFU-C colony proliferation. Addition of exogenous IL2 caused an increase in CFU-C colony numbers in a dose-dependent manner. This increase could be prevented by anti-Tac, a monoclonal antibody (MoAb) to the IL2 receptor. Moreover, anti-Tac in the absence of exogenous IL2 resulted in an overall decrease in colony numbers. Depletion of either adherent cells or T lymphocytes abolished the effect of IL2 and anti-Tac on colony growth. In the presence of IL2, re-addition of T lymphocytes to the T-depleted marrow or adherent cells to adherent cell-depleted marrow resulted in a significant increase in CFU-C colony numbers, whereas no significant effect was found when IL2-depleted CSA was used. Although T lymphocytes were not themselves essential for CFU-C colony growth, our studies indicate that IL2 and IL2-responsive T cells can regulate in vitro granulopoiesis.

AB - The effect of recombinant interleukin 2 (IL2) on marrow CFU-C colony formation was evaluated to define the role for T lymphocytes in human marrow granulopoiesis. The colony-stimulating factor (CSA) used in our experiments was found to contain IL2. IL2 depletion from CSA resulted in a reduction in CFU-C colony proliferation. Addition of exogenous IL2 caused an increase in CFU-C colony numbers in a dose-dependent manner. This increase could be prevented by anti-Tac, a monoclonal antibody (MoAb) to the IL2 receptor. Moreover, anti-Tac in the absence of exogenous IL2 resulted in an overall decrease in colony numbers. Depletion of either adherent cells or T lymphocytes abolished the effect of IL2 and anti-Tac on colony growth. In the presence of IL2, re-addition of T lymphocytes to the T-depleted marrow or adherent cells to adherent cell-depleted marrow resulted in a significant increase in CFU-C colony numbers, whereas no significant effect was found when IL2-depleted CSA was used. Although T lymphocytes were not themselves essential for CFU-C colony growth, our studies indicate that IL2 and IL2-responsive T cells can regulate in vitro granulopoiesis.

UR - http://www.scopus.com/inward/record.url?scp=0023237535&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023237535&partnerID=8YFLogxK

M3 - Article

VL - 69

SP - 1161

EP - 1166

JO - Blood

JF - Blood

SN - 0006-4971

IS - 4

ER -