The regulatory role of Interleukin 2-responsive T lymphocytes on human marrow granulopoiesis

Z. Estrov, C. Roifman, G. Mills, T. Grunberger, E. W. Gelfand, M. H. Freedman

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    Abstract

    The effect of recombinant interleukin 2 (IL2) on marrow CFU-C colony formation was evaluated to define the role for T lymphocytes in human marrow granulopoiesis. The colony-stimulating factor (CSA) used in our experiments was found to contain IL2. IL2 depletion from CSA resulted in a reduction in CFU-C colony proliferation. Addition of exogenous IL2 caused an increase in CFU-C colony numbers in a dose-dependent manner. This increase could be prevented by anti-Tac, a monoclonal antibody (MoAb) to the IL2 receptor. Moreover, anti-Tac in the absence of exogenous IL2 resulted in an overall decrease in colony numbers. Depletion of either adherent cells or T lymphocytes abolished the effect of IL2 and anti-Tac on colony growth. In the presence of IL2, re-addition of T lymphocytes to the T-depleted marrow or adherent cells to adherent cell-depleted marrow resulted in a significant increase in CFU-C colony numbers, whereas no significant effect was found when IL2-depleted CSA was used. Although T lymphocytes were not themselves essential for CFU-C colony growth, our studies indicate that IL2 and IL2-responsive T cells can regulate in vitro granulopoiesis.

    Original languageEnglish (US)
    Pages (from-to)1161-1166
    Number of pages6
    JournalBlood
    Volume69
    Issue number4
    StatePublished - Jul 30 1987

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    ASJC Scopus subject areas

    • Biochemistry
    • Immunology
    • Hematology
    • Cell Biology

    Cite this

    Estrov, Z., Roifman, C., Mills, G., Grunberger, T., Gelfand, E. W., & Freedman, M. H. (1987). The regulatory role of Interleukin 2-responsive T lymphocytes on human marrow granulopoiesis. Blood, 69(4), 1161-1166.