The recognition site of type II restriction enzyme BglI is interrupted

James A. Lautenberger, C. Thomas White, Nancy Haigwood, Marshall H. Edgell, Clyde A. Hutchison

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The Type II restriction endonuclease BglI recognizes the interrupted DNA sequences 5′-G-C-C-N-N-N-N-N-G-G-C-. This sequence occurs at all locations in over 33 000 base pairs of DNA sequence where the enzyme was found to cut DNA and nowhere else. All six of the specified bases are essential parts of the site since all groups of five of the six bases occur in the DNA sequences tested and none of them are cut by BglI. The length of the block of intervening unspecified positions must be exactly five since all other sizes between zero and 15 occur in the DNA sequences searched and none are cut by BglI. The 5′-terminal nucleotides of BglI cleaved phage G4 replicative form DNA and plasmid pER18 DNA were compared with the DNA sequences near the BglI sites on these DNAs. These results indicated that BglI cuts within the intervening unspecified region and produces single-stranded 3′ termini that are three bases long. The BglI recognition site and cleavage points can thus be represented as follows: 5′-G-C-C-N-N-N-N-N-G-G-C-3′3′-C-G-G-N-N-N-N-N-C-C-G-5′ This study of the BglI recognition site was facilitated by the use of inexpensive microcomputers. A system of programs was developed that allowed analysis of over 33 kb of DNA sequences stored on flexible magnetic disks or audio cassettes. While these programs were generally written in the higher level language BASIC, some assembly language subroutines were utilized to reduce execution time.

Original languageEnglish (US)
Pages (from-to)213-231
Number of pages19
JournalGene
Volume9
Issue number3-4
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Type II Site Specific Deoxyribonucleases
DNA
Microvirus
Language
Microcomputers
Base Pairing
Plasmids
Nucleotides
Enzymes

Keywords

  • DNA sequence data base
  • microcomputers
  • symmetrical interrupted hexanucleotide
  • Variable sticky ends

ASJC Scopus subject areas

  • Genetics

Cite this

Lautenberger, J. A., Thomas White, C., Haigwood, N., Edgell, M. H., & Hutchison, C. A. (1990). The recognition site of type II restriction enzyme BglI is interrupted. Gene, 9(3-4), 213-231. https://doi.org/10.1016/0378-1119(90)90324-K

The recognition site of type II restriction enzyme BglI is interrupted. / Lautenberger, James A.; Thomas White, C.; Haigwood, Nancy; Edgell, Marshall H.; Hutchison, Clyde A.

In: Gene, Vol. 9, No. 3-4, 1990, p. 213-231.

Research output: Contribution to journalArticle

Lautenberger, JA, Thomas White, C, Haigwood, N, Edgell, MH & Hutchison, CA 1990, 'The recognition site of type II restriction enzyme BglI is interrupted', Gene, vol. 9, no. 3-4, pp. 213-231. https://doi.org/10.1016/0378-1119(90)90324-K
Lautenberger, James A. ; Thomas White, C. ; Haigwood, Nancy ; Edgell, Marshall H. ; Hutchison, Clyde A. / The recognition site of type II restriction enzyme BglI is interrupted. In: Gene. 1990 ; Vol. 9, No. 3-4. pp. 213-231.
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AB - The Type II restriction endonuclease BglI recognizes the interrupted DNA sequences 5′-G-C-C-N-N-N-N-N-G-G-C-. This sequence occurs at all locations in over 33 000 base pairs of DNA sequence where the enzyme was found to cut DNA and nowhere else. All six of the specified bases are essential parts of the site since all groups of five of the six bases occur in the DNA sequences tested and none of them are cut by BglI. The length of the block of intervening unspecified positions must be exactly five since all other sizes between zero and 15 occur in the DNA sequences searched and none are cut by BglI. The 5′-terminal nucleotides of BglI cleaved phage G4 replicative form DNA and plasmid pER18 DNA were compared with the DNA sequences near the BglI sites on these DNAs. These results indicated that BglI cuts within the intervening unspecified region and produces single-stranded 3′ termini that are three bases long. The BglI recognition site and cleavage points can thus be represented as follows: 5′-G-C-C-N-N-N-N↓-N-G-G-C-3′3′-C-G-G-N↑-N-N-N-N-C-C-G-5′ This study of the BglI recognition site was facilitated by the use of inexpensive microcomputers. A system of programs was developed that allowed analysis of over 33 kb of DNA sequences stored on flexible magnetic disks or audio cassettes. While these programs were generally written in the higher level language BASIC, some assembly language subroutines were utilized to reduce execution time.

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