The proteome of mouse vestibular hair bundles over development

Jocelyn F. Krey, Nicholas E. Sherman, Erin D. Jeffery, Dongseok Choi, Peter Barr-Gillespie

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Development of the vertebrate hair bundle is a precisely orchestrated event that culminates in production of a tightly ordered arrangement of actin-rich stereocilia and a single axonemal kinocilium. To understand how the protein composition of the bundle changes during development, we isolated bundles from young (postnatal days P4-P6) and mature (P21-P25) mouse utricles using the twist-off method, then characterized their constituent proteins using liquid-chromatography tandem mass spectrometry with data-dependent acquisition. Using MaxQuant and label-free quantitation, we measured relative abundances of proteins in both bundles and in the whole utricle; comparison of protein abundance between the two fractions allows calculation of enrichment in bundles. These data, which are available via ProteomeXchange with identifier PXD002167, will be useful for examining the proteins present in mammalian vestibular bundles and how their concentrations change over development.

Original languageEnglish (US)
Article number150047
JournalScientific data
Volume2
DOIs
StatePublished - Sep 15 2015

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Mouse
Bundle
Proteins
Protein
data acquisition
Liquid chromatography
event
Actin
present
Dependent Data
Mass Spectrometry
Mass spectrometry
Labels
Chromatography
Twist
Arrangement
Liquid
Chemical analysis

ASJC Scopus subject areas

  • Education
  • Library and Information Sciences
  • Computer Science Applications
  • Information Systems
  • Statistics, Probability and Uncertainty
  • Statistics and Probability

Cite this

The proteome of mouse vestibular hair bundles over development. / Krey, Jocelyn F.; Sherman, Nicholas E.; Jeffery, Erin D.; Choi, Dongseok; Barr-Gillespie, Peter.

In: Scientific data, Vol. 2, 150047, 15.09.2015.

Research output: Contribution to journalArticle

Krey, Jocelyn F. ; Sherman, Nicholas E. ; Jeffery, Erin D. ; Choi, Dongseok ; Barr-Gillespie, Peter. / The proteome of mouse vestibular hair bundles over development. In: Scientific data. 2015 ; Vol. 2.
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