The potential of human allogeneic juvenile chondrocytes for restoration of articular cartilage

H. Davis Adkisson, James A. Martin, Richard L. Amendola, Curt Milliman, Kelsey A. Mauch, Arbindra B. Katwal, Mitchell Seyedin, Annuziato Amendola, Philip Streeter, Joseph A. Buckwalter

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Background: Donor-site morbidity, limited numbers of cells, loss of phenotype during ex vivo expansion, and age-related decline in chondrogenic activity present critical obstacles to the use of autologous chondrocyte implantation for cartilage repair. Chondrocytes from juvenile cadaveric donors may represent an alternative to autologous cells. Hypothesis/Purpose: The authors hypothesized that juvenile chondrocyte would show stronger and more stable chondrogenic activity than adult cells in vitro and that juvenile cells pose little risk of immunologic incompatibility in adult hosts. Study Design: Controlled laboratory study. Methods: Cartilage samples were from juvenile (13 years old) donors. The chondrogenic activity of freshly isolated human articular chondrocytes and of expanded cells after monolayer culture was measured by proteoglycan assay, gene expression analysis, and histology. Lymphocyte proliferation assays were used to assess immunogenic activity. Results: Proteoglycan content in neocartilage produced by juvenile chondrocytes was 100-fold higher than in neocartilage produced by adult cells. Collagen type II and type IX mRNA in fresh juvenile chondrocytes were 100- and 700-fold higher, respectively, than in adult chondrocytes. The distributions of collagens II and IX were similar in native juvenile cartilage and in neocartilage made by juvenile cells. Juvenile cells grew significantly faster in monolayer cultures than adult cells (P =.002) and proteoglycan levels produced in agarose culture was significantly higher in juvenile cells than in adult cells after multiple passages (P

Original languageEnglish (US)
Pages (from-to)1324-1333
Number of pages10
JournalAmerican Journal of Sports Medicine
Volume38
Issue number7
DOIs
StatePublished - Jul 2010

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Articular Cartilage
Chondrocytes
Proteoglycans
Cartilage
Collagen Type II
Sepharose
Histology
Collagen
Cell Culture Techniques
Cell Count
Joints
Lymphocytes
Morbidity
Phenotype
Gene Expression
Messenger RNA

Keywords

  • agarose culture
  • aging
  • cartilage repair
  • chondrocyte transplantation
  • immunogenicity
  • juvenile human chondrocytes
  • neocartilage
  • serial expansion

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation
  • Medicine(all)

Cite this

Adkisson, H. D., Martin, J. A., Amendola, R. L., Milliman, C., Mauch, K. A., Katwal, A. B., ... Buckwalter, J. A. (2010). The potential of human allogeneic juvenile chondrocytes for restoration of articular cartilage. American Journal of Sports Medicine, 38(7), 1324-1333. https://doi.org/10.1177/0363546510361950

The potential of human allogeneic juvenile chondrocytes for restoration of articular cartilage. / Adkisson, H. Davis; Martin, James A.; Amendola, Richard L.; Milliman, Curt; Mauch, Kelsey A.; Katwal, Arbindra B.; Seyedin, Mitchell; Amendola, Annuziato; Streeter, Philip; Buckwalter, Joseph A.

In: American Journal of Sports Medicine, Vol. 38, No. 7, 07.2010, p. 1324-1333.

Research output: Contribution to journalArticle

Adkisson, HD, Martin, JA, Amendola, RL, Milliman, C, Mauch, KA, Katwal, AB, Seyedin, M, Amendola, A, Streeter, P & Buckwalter, JA 2010, 'The potential of human allogeneic juvenile chondrocytes for restoration of articular cartilage', American Journal of Sports Medicine, vol. 38, no. 7, pp. 1324-1333. https://doi.org/10.1177/0363546510361950
Adkisson, H. Davis ; Martin, James A. ; Amendola, Richard L. ; Milliman, Curt ; Mauch, Kelsey A. ; Katwal, Arbindra B. ; Seyedin, Mitchell ; Amendola, Annuziato ; Streeter, Philip ; Buckwalter, Joseph A. / The potential of human allogeneic juvenile chondrocytes for restoration of articular cartilage. In: American Journal of Sports Medicine. 2010 ; Vol. 38, No. 7. pp. 1324-1333.
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