The polymerase chain reaction for the detection of Borrelia burgdorferi in human body fluids

Michael Liebling, Midori Jane Nishio, Annette Rodriguez, Leonard H. Sigal, Tian Jin, James S. Louie

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Objective. To analyze clinical fluids for the presence of Borrelia burgdorferi DNA using the polymerase chain reaction (PCR). Methods. We utilized a modified, nested PCR to detect the presence of Borrelia DNA in 99 samples of serum, urine, cerebrospinal fluid (CSF), or synovial fluid obtained from 44 patients with various stages of Lyme disease and 47 control subjects. Primer specificity was corroborated by examining 2 DNA data banks, testing against DNA from other organisms, and confirming results with a second set of nested primers. Results. Nested PCR was capable of detecting DNA from fewer than 10 organisms in 1 ml of fluid. The specificity of this technique was 96.4%, with a sensitivity of 76.7%. Although the specificity was uniformly high, the sensitivity was dependent upon the body fluid being tested: CSF 100%, urine 100%, synovial fluid 80%, and serum 59%. The rate of false-positive results was 3.6%. Conclusion. These data demonstrate the potential utility of PCR in confirming the clinical diagnosis of Lyme disease as well as providing insight into the pathogenesis of various stages of this disorder.

Original languageEnglish (US)
Pages (from-to)665-675
Number of pages11
JournalArthritis and Rheumatism
Volume36
Issue number5
StatePublished - May 1993
Externally publishedYes

Fingerprint

Borrelia burgdorferi
Body Fluids
Human Body
Polymerase Chain Reaction
Lyme Disease
Synovial Fluid
Cerebrospinal Fluid
DNA
Urine
Borrelia
Nucleic Acid Databases
DNA-Directed DNA Polymerase
Serum

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Liebling, M., Nishio, M. J., Rodriguez, A., Sigal, L. H., Jin, T., & Louie, J. S. (1993). The polymerase chain reaction for the detection of Borrelia burgdorferi in human body fluids. Arthritis and Rheumatism, 36(5), 665-675.

The polymerase chain reaction for the detection of Borrelia burgdorferi in human body fluids. / Liebling, Michael; Nishio, Midori Jane; Rodriguez, Annette; Sigal, Leonard H.; Jin, Tian; Louie, James S.

In: Arthritis and Rheumatism, Vol. 36, No. 5, 05.1993, p. 665-675.

Research output: Contribution to journalArticle

Liebling, M, Nishio, MJ, Rodriguez, A, Sigal, LH, Jin, T & Louie, JS 1993, 'The polymerase chain reaction for the detection of Borrelia burgdorferi in human body fluids', Arthritis and Rheumatism, vol. 36, no. 5, pp. 665-675.
Liebling M, Nishio MJ, Rodriguez A, Sigal LH, Jin T, Louie JS. The polymerase chain reaction for the detection of Borrelia burgdorferi in human body fluids. Arthritis and Rheumatism. 1993 May;36(5):665-675.
Liebling, Michael ; Nishio, Midori Jane ; Rodriguez, Annette ; Sigal, Leonard H. ; Jin, Tian ; Louie, James S. / The polymerase chain reaction for the detection of Borrelia burgdorferi in human body fluids. In: Arthritis and Rheumatism. 1993 ; Vol. 36, No. 5. pp. 665-675.
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