TY - JOUR
T1 - The polarized sorting of membrane proteins expressed in cultured hippocampal neurons using viral vectors
AU - Jareb, Mark
AU - Banker, Gary
N1 - Funding Information:
This research was supported by a grant from the National Institutes of Health (NS17112). M. J. was supported in part by an NIH training grant (HD07323). We thank Hannelore Asmussen for the preparation of neuronal cultures; all those who generously gave cDNA, viruses, or antibodies; and Jim Casanova, Ira Mellman, Peter Sonderegger, Lorenz Vogt, and Michelle Burack for their helpful discussion and critical comments. We also thank the Banker lab for their valuable advice and encouragement throughout the course of this work.
PY - 1998/5
Y1 - 1998/5
N2 - One model of neuronal polarity (Dotti and Simons, 1990) proposes that neurons and polarized epithelia use similar mechanisms to sort membrane proteins. To explore this hypothesis, we used viral vectors to express proteins in cultured neurons and assessed their distribution using quantitative immunofluorescence microscopy. Basolateral epithelial proteins were polarized to dendrites; more significantly, mutations of sequences required for their basolateral targeting in epithelia also disrupted dendritic targeting. Unexpectedly, apical proteins were not polarized to axons but were expressed at roughly equal amounts in dendrites and axons. These data provide strong evidence that targeting of basolateral and dendritic proteins depends on common mechanisms. In contrast, the sorting of proteins to the axon requires signals that are not present in apical proteins.
AB - One model of neuronal polarity (Dotti and Simons, 1990) proposes that neurons and polarized epithelia use similar mechanisms to sort membrane proteins. To explore this hypothesis, we used viral vectors to express proteins in cultured neurons and assessed their distribution using quantitative immunofluorescence microscopy. Basolateral epithelial proteins were polarized to dendrites; more significantly, mutations of sequences required for their basolateral targeting in epithelia also disrupted dendritic targeting. Unexpectedly, apical proteins were not polarized to axons but were expressed at roughly equal amounts in dendrites and axons. These data provide strong evidence that targeting of basolateral and dendritic proteins depends on common mechanisms. In contrast, the sorting of proteins to the axon requires signals that are not present in apical proteins.
UR - http://www.scopus.com/inward/record.url?scp=0032078142&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032078142&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(00)80468-7
DO - 10.1016/S0896-6273(00)80468-7
M3 - Article
C2 - 9620691
AN - SCOPUS:0032078142
SN - 0896-6273
VL - 20
SP - 855
EP - 867
JO - Neuron
JF - Neuron
IS - 5
ER -