The peritoneal cavity B-2 antibody repertoire appears to reflect many of the same selective pressures that shape the B-1a and B-1b repertoires

Andre M. Vale, Jason M. Tanner, Robert Schelonka, Yingxin Zhuang, Michael Zemlin, G. Larry Gartland, Harry W. Schroeder

Research output: Contribution to journalArticle

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Abstract

To assess the extent and nature of somatic categorical selection of CDR-3 of the Ig H chain (CDR-H3) content in peritoneal cavity (PerC) B cells, we analyzed the composition of VH7183DJCμ transcripts derived from sorted PerC B-1a, B-1b, and B-2 cells. We divided these sequences into those that contained N nucleotides (N+) and those that did not (N -) and then compared them with sequences cloned from sorted IgM +IgD+ B cells from neonatal liver and both wild-type and TdT-deficient adult bone marrow. We found that the PerC B-1a N- repertoire is enriched for the signatures of CDR-H3 sequences present in neonatal liver and shares many features with the B-1b N- repertoire, whereas the PerC B-1a N+, B-1b N+, and B-2 N+ repertoires are enriched for adult bone marrow sequence signatures. However, we also found several sequence signatures that were not shared with other mature perinatal or adult B cell subsets but were either unique or variably shared between the two or even among all three of the PerC subsets that we examined. These signatures included more sequences lacking N nucleotides in the B-2 population and an increased use of DH reading frame 2, which created CDR-H3s of greater average hydrophobicity. These findings provide support for both ontogenetic origin and shared Ag receptor-influenced selection as the mechanisms that shape the unique composition of the B-1a, B-1b, and B-2 repertoires. The PerC may thus serve as a general reservoir for B cells with Ag binding specificities that are uncommon in other mature compartments.

Original languageEnglish (US)
Pages (from-to)6085-6095
Number of pages11
JournalJournal of Immunology
Volume185
Issue number10
DOIs
StatePublished - Nov 15 2010

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Peritoneal Cavity
Antibodies
B-Lymphocytes
Nucleotides
Bone Marrow
B-Lymphocyte Subsets
Immunoglobulin D
Reading Frames
Liver
Hydrophobic and Hydrophilic Interactions
Immunoglobulin M
Population

ASJC Scopus subject areas

  • Immunology

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The peritoneal cavity B-2 antibody repertoire appears to reflect many of the same selective pressures that shape the B-1a and B-1b repertoires. / Vale, Andre M.; Tanner, Jason M.; Schelonka, Robert; Zhuang, Yingxin; Zemlin, Michael; Gartland, G. Larry; Schroeder, Harry W.

In: Journal of Immunology, Vol. 185, No. 10, 15.11.2010, p. 6085-6095.

Research output: Contribution to journalArticle

Vale, Andre M. ; Tanner, Jason M. ; Schelonka, Robert ; Zhuang, Yingxin ; Zemlin, Michael ; Gartland, G. Larry ; Schroeder, Harry W. / The peritoneal cavity B-2 antibody repertoire appears to reflect many of the same selective pressures that shape the B-1a and B-1b repertoires. In: Journal of Immunology. 2010 ; Vol. 185, No. 10. pp. 6085-6095.
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