The peritoneal cavity B-2 antibody repertoire appears to reflect many of the same selective pressures that shape the B-1a and B-1b repertoires

Andre M. Vale, Jason M. Tanner, Robert L. Schelonka, Yingxin Zhuang, Michael Zemlin, G. Larry Gartland, Harry W. Schroeder

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

To assess the extent and nature of somatic categorical selection of CDR-3 of the Ig H chain (CDR-H3) content in peritoneal cavity (PerC) B cells, we analyzed the composition of VH7183DJCμ transcripts derived from sorted PerC B-1a, B-1b, and B-2 cells. We divided these sequences into those that contained N nucleotides (N+) and those that did not (N -) and then compared them with sequences cloned from sorted IgM +IgD+ B cells from neonatal liver and both wild-type and TdT-deficient adult bone marrow. We found that the PerC B-1a N- repertoire is enriched for the signatures of CDR-H3 sequences present in neonatal liver and shares many features with the B-1b N- repertoire, whereas the PerC B-1a N+, B-1b N+, and B-2 N+ repertoires are enriched for adult bone marrow sequence signatures. However, we also found several sequence signatures that were not shared with other mature perinatal or adult B cell subsets but were either unique or variably shared between the two or even among all three of the PerC subsets that we examined. These signatures included more sequences lacking N nucleotides in the B-2 population and an increased use of DH reading frame 2, which created CDR-H3s of greater average hydrophobicity. These findings provide support for both ontogenetic origin and shared Ag receptor-influenced selection as the mechanisms that shape the unique composition of the B-1a, B-1b, and B-2 repertoires. The PerC may thus serve as a general reservoir for B cells with Ag binding specificities that are uncommon in other mature compartments.

Original languageEnglish (US)
Pages (from-to)6085-6095
Number of pages11
JournalJournal of Immunology
Volume185
Issue number10
DOIs
StatePublished - Nov 15 2010

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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