The Orphan Nuclear Receptor Small Heterodimer Partner as a Novel Coregulator of Nuclear Factor-κB in Oxidized Low Density Lipoprotein-treated Macrophage Cell Line RAW 264.7

Yun Sung Kim, Chang Yeop Han, Seung Whan Kim, Jung Hyun Kim, Soo Kyung Lee, Dong Ju Jung, Soo Young Park, Heonjoong Kang, Hueng Sik Choi, Jae Woon Lee, Youngmi Kim Pak

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45 Scopus citations


Small heterodimer partner (SHP), specifically expressed in liver and a limited number of other tissues, is an unusual orphan nuclear receptor that lacks the conventional DNA binding domain. In this work, we found that SHP expression is abundant in murine macrophage cell line RAW 264.7 but was suppressed by oxidized low density lipoprotein (oxLDL) and its constituent 13-hydroxyoctadecadienoic acid, a ligand for peroxisome proliferator-activated receptor γ. Furthermore, SHP acted as a transcription coactivator of nuclear factor-κB (NFκB) and was essential for the previously described NFκB transactivation by palmitoyl lysophosphatidylcholine, one of the oxLDL constituents. Accordingly NFκB, which was transcriptionally active in the beginning, became progressively inert in oxLDL-treated RAW 264.7 cells as oxLDL decreased the SHP expression. Thus, SHP appears to be an important modulatory component to regulate the transcriptional activities of NFκB in oxLDL-treated, resting macrophage cells.

Original languageEnglish (US)
Pages (from-to)33736-33740
Number of pages5
JournalJournal of Biological Chemistry
Issue number36
StatePublished - Sep 7 2001


ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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