The nuclear accumulation of the Fanconi anemia protein FANCE depends on FANCC

France Léveillé, Miriam Ferrer, Annette L. Medhurst, El Houari Laghmani, Martin A. Rooimans, Patrick Bier, Jurgen Steltenpool, Tom A. Titus, John H. Postlethwait, Maureen E. Hoatlin, Hans Joenje, Johan P. de Winter

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The Fanconi anemia (FA) protein FANCE is an essential component of the nuclear FA core complex, which is required for monoubiquitination of the downstream target FANCD2, an important step in the FA pathway of DNA cross-link repair. FANCE is predominantly localized in the nucleus and acts as a molecular bridge between the FA core complex and FANCD2, through direct binding of both FANCC and FANCD2. At present, it is poorly understood how the nuclear accumulation of FANCE is regulated and therefore we investigated the nuclear localization of this FA protein. We found that FANCE has a strong tendency to localize in the nucleus, since the addition of a nuclear export signal does not interfere with the nuclear localization of FANCE. We also demonstrate that the nuclear accumulation of FANCE does not rely solely on its nuclear localization signal motifs, but also on FANCC. The other FA proteins are not involved in the nuclear accumulation of FANCE, indicating a tight relationship between FANCC and FANCE, as suggested from their direct interaction. Finally, we show that the region of FANCE interacting with FANCC appears to be different from the region involved in binding FANCD2. This strengthens the idea that FANCE recruits FANCD2 to the core complex, without interfering with the binding of FANCC.

Original languageEnglish (US)
Pages (from-to)556-565
Number of pages10
JournalDNA Repair
Volume5
Issue number5
DOIs
StatePublished - May 10 2006

Keywords

  • FANCC
  • FANCD2
  • FANCE
  • Fanconi anemia
  • Interaction
  • Nuclear accumulation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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