The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression

Michiko Nakano, Hao Geng, Shunji Nakano, Kazuo Kobayashi

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The ResD-ResE signal transduction system is essential for aerobic and anaerobic respiration in Bacillus subtilis. ResDE-dependent gene expression is induced by oxygen limitation, but full induction under anaerobic conditions requires nitrite or nitric oxide (NO). Here we report that NsrR (formerly YhdE) is responsible for the NO-dependent up-regulation of the ResDE regulon. The null mutation of nsrR led to aerobic derepression of hmp (flavohemoglobin gene) partly in a ResDE-independent manner. In addition to its negative role in aerobic hmp expression, NsrR plays an important role under anaerobic conditions for regulation of ResDE-controlled genes, including hmp. ResDE-dependent gene expression was increased by the nsrR mutation in the absence of NO, but the expression was decreased by the mutation when NO was present. Consequently, B. subtilis cells lacking NsrR no longer sense and respond to NO (and nitrite) to up-regulate the ResDE regulon. Exposure to NO did not significantly change the cellular concentration of NsrR, suggesting that NO likely modulates the activity of NsrR. NsrR is similar to the recently described nitrite- or NO-sensitive transcription repressers present in various bacteria. NsrR likely has an Fe-S cluster, and interaction of NO with the Fe-S center is proposed to modulate NsrR activity.

Original languageEnglish (US)
Pages (from-to)5878-5887
Number of pages10
JournalJournal of Bacteriology
Volume188
Issue number16
DOIs
StatePublished - Aug 2006

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Nitric Oxide
Gene Expression
Nitrites
Regulon
Bacillus subtilis
Mutation
Up-Regulation
Genes
Signal Transduction
Respiration
Oxygen
Bacteria

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression. / Nakano, Michiko; Geng, Hao; Nakano, Shunji; Kobayashi, Kazuo.

In: Journal of Bacteriology, Vol. 188, No. 16, 08.2006, p. 5878-5887.

Research output: Contribution to journalArticle

Nakano, Michiko ; Geng, Hao ; Nakano, Shunji ; Kobayashi, Kazuo. / The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression. In: Journal of Bacteriology. 2006 ; Vol. 188, No. 16. pp. 5878-5887.
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