The Neisseria meningitidis haemoglobin receptor

Its role in iron utilization and virulence

Igor Stojiljkovic, Vivian Hwa, Luc De Saint Martin, Peadar O'Gaora, Xavier Nassif, Fred Heffron, Magdalene So

    Research output: Contribution to journalArticle

    157 Citations (Scopus)

    Abstract

    The Neisseria meningitidis haemoglobin receptor gene, hmbR, was cloned by complementation in a porphyrin-requiring Escherichia coli mutant. hmbR encodes an 89.5 kDa outer membrane protein which shares amino acid homology with the TonB-dependent receptors of Gram-negative bacteria. HmbR had the highest similarity to Neisseria transferrin and lactoferrin receptors. The utilization of haemoglobin as an iron source required internalization of the haemin moiety by the cell. The mechanism of haemin internalization via the haemoglobin receptor was TonB-dependent in E. coli. A N. meningitidis hmbR mutant was unable to use haemoglobin but could still use haemin as a sole iron source. The existence of a second N. meningitidis receptor gene, specific for haemin, was shown by the isolation of cosmids which did not hybridize with the hmbR probe, but which were able to complement an E. coli hemA aroB mutant on haemin-supplemented plates. The N. meningitidis hmbR mutant was attenuated in an infant rat model for meningococcal infection, indicating that haemoglobin utilization is important for N. meningitidis virulence.

    Original languageEnglish (US)
    Pages (from-to)531-541
    Number of pages11
    JournalMolecular Microbiology
    Volume15
    Issue number3
    DOIs
    StatePublished - Feb 1995

    Fingerprint

    Hemin
    Neisseria meningitidis
    Virulence
    Iron
    Hemoglobins
    Escherichia coli
    Meningococcal Infections
    Neisseria
    Cosmids
    Transferrin Receptors
    Porphyrins
    Gram-Negative Bacteria
    Genes
    Membrane Proteins
    Neisseria meningitidis HmbR protein
    Amino Acids

    ASJC Scopus subject areas

    • Molecular Biology
    • Microbiology

    Cite this

    Stojiljkovic, I., Hwa, V., De Saint Martin, L., O'Gaora, P., Nassif, X., Heffron, F., & So, M. (1995). The Neisseria meningitidis haemoglobin receptor: Its role in iron utilization and virulence. Molecular Microbiology, 15(3), 531-541. https://doi.org/10.1111/j.1365-2958.1995.tb02266.x

    The Neisseria meningitidis haemoglobin receptor : Its role in iron utilization and virulence. / Stojiljkovic, Igor; Hwa, Vivian; De Saint Martin, Luc; O'Gaora, Peadar; Nassif, Xavier; Heffron, Fred; So, Magdalene.

    In: Molecular Microbiology, Vol. 15, No. 3, 02.1995, p. 531-541.

    Research output: Contribution to journalArticle

    Stojiljkovic, I, Hwa, V, De Saint Martin, L, O'Gaora, P, Nassif, X, Heffron, F & So, M 1995, 'The Neisseria meningitidis haemoglobin receptor: Its role in iron utilization and virulence', Molecular Microbiology, vol. 15, no. 3, pp. 531-541. https://doi.org/10.1111/j.1365-2958.1995.tb02266.x
    Stojiljkovic, Igor ; Hwa, Vivian ; De Saint Martin, Luc ; O'Gaora, Peadar ; Nassif, Xavier ; Heffron, Fred ; So, Magdalene. / The Neisseria meningitidis haemoglobin receptor : Its role in iron utilization and virulence. In: Molecular Microbiology. 1995 ; Vol. 15, No. 3. pp. 531-541.
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