The N-terminal domain of p73 interacts with the CH1 domain of p300/CREB binding protein and mediates transcriptional activation and apoptosis

Xiaoya Zeng, Xiaorong Li, Ashley Miller, Zhimin Yuan, Wuchao Yuan, Roland P S Kwok, Richard Goodman, Hua Lu

Research output: Contribution to journalArticle

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Abstract

The newly identified p53 homolog p73 mimics the transcriptional function of p53. We have investigated the regulation of p73's transcriptional activity by p300/CREB binding protein (CBP). p73-p300 complexes were identified in HeLa cell extracts by cofractionation and coimmunoprecipitation assays. The p73-p300 interaction was confirmed in vitro by glutathione S-transferase- protein association assays and in vivo by coimmunoprecipitating the overexpressed p300 and p73 in human p53-free small-cell lung carcinoma H1299 or osteosarcoma Saos-2 cells. The N terminus but not the N-terminal truncation of p73 bound to the CH1 domain (amino acids [aa] 350 to 450) of p300/CBP. Accordingly, this p73 N-terminal deletion was unable to activate transcription or to induce apoptosis. Overexpression of either p300 or CBP stimulated transcription mediated by p73 but not its N-terminally deleted mutant in vivo. The N-terminal fragment from aa 19 to 597, but not the truncated fragment from aa 242 to 1700 of p300, reduced p73-mediated transcription markedly, p73-dependent transcription or apoptosis was partially impaired in either p300- or CBP-deficient human breast carcinoma MCF-7 or H1299 cells, suggesting that both coactivators mediate transcription by p73 in cells. These results demonstrate that the N terminus of p73 directly interacts with the N-terminal CH1 domain of p300/CBP to activate transcription.

Original languageEnglish (US)
Pages (from-to)1299-1310
Number of pages12
JournalMolecular and Cellular Biology
Volume20
Issue number4
DOIs
StatePublished - Feb 2000

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CREB-Binding Protein
Transcriptional Activation
Apoptosis
Amino Acids
Small Cell Lung Carcinoma
Osteosarcoma
Cell Extracts
Glutathione Transferase
HeLa Cells
Breast Neoplasms
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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The N-terminal domain of p73 interacts with the CH1 domain of p300/CREB binding protein and mediates transcriptional activation and apoptosis. / Zeng, Xiaoya; Li, Xiaorong; Miller, Ashley; Yuan, Zhimin; Yuan, Wuchao; Kwok, Roland P S; Goodman, Richard; Lu, Hua.

In: Molecular and Cellular Biology, Vol. 20, No. 4, 02.2000, p. 1299-1310.

Research output: Contribution to journalArticle

Zeng, Xiaoya ; Li, Xiaorong ; Miller, Ashley ; Yuan, Zhimin ; Yuan, Wuchao ; Kwok, Roland P S ; Goodman, Richard ; Lu, Hua. / The N-terminal domain of p73 interacts with the CH1 domain of p300/CREB binding protein and mediates transcriptional activation and apoptosis. In: Molecular and Cellular Biology. 2000 ; Vol. 20, No. 4. pp. 1299-1310.
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abstract = "The newly identified p53 homolog p73 mimics the transcriptional function of p53. We have investigated the regulation of p73's transcriptional activity by p300/CREB binding protein (CBP). p73-p300 complexes were identified in HeLa cell extracts by cofractionation and coimmunoprecipitation assays. The p73-p300 interaction was confirmed in vitro by glutathione S-transferase- protein association assays and in vivo by coimmunoprecipitating the overexpressed p300 and p73 in human p53-free small-cell lung carcinoma H1299 or osteosarcoma Saos-2 cells. The N terminus but not the N-terminal truncation of p73 bound to the CH1 domain (amino acids [aa] 350 to 450) of p300/CBP. Accordingly, this p73 N-terminal deletion was unable to activate transcription or to induce apoptosis. Overexpression of either p300 or CBP stimulated transcription mediated by p73 but not its N-terminally deleted mutant in vivo. The N-terminal fragment from aa 19 to 597, but not the truncated fragment from aa 242 to 1700 of p300, reduced p73-mediated transcription markedly, p73-dependent transcription or apoptosis was partially impaired in either p300- or CBP-deficient human breast carcinoma MCF-7 or H1299 cells, suggesting that both coactivators mediate transcription by p73 in cells. These results demonstrate that the N terminus of p73 directly interacts with the N-terminal CH1 domain of p300/CBP to activate transcription.",
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