The murine cytomegalovirus immunomodulatory gene m152 prevents recognition of infected cells by M45-specific CTL but does not alter the immunodominance of the M45-specific CD8 T cell response in vivo

Marielle Gold, Michael Munks, Markus Wagner, Ulrich H. Koszinowski, Ann Hill, Steven P. Fling

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Although in vitro studies have shown that herpesviruses, including murine CMV (MCMV), encode genes that interfere with the MHC class I pathway, their effects on the CTL response in vivo is unclear. We identified a Db-restricted CTL epitope from MCMV M45 by screening an MCMV genomic library using CTL clones isolated from mice infected with MCMV lacking m152. Because m152 severely inhibits CTL recognition of M45 in vitro, we questioned whether an M45-specific response would be generated in mice infected with wild-type MCMV expressing m152. Mice infected with wild-type MCMV or MCMVΔm152 made similar responses to the M45 Ag. Moreover, we saw no skewing of the proportion of M45-specific CD8 T cells within the total MCMV-specific response after infection with MCMV with m152. Despite the profound effect m152 has on presentation of M45 in vitro, it does not affect the immunodominance of M45 in the CTL response in vivo.

Original languageEnglish (US)
Pages (from-to)359-365
Number of pages7
JournalJournal of Immunology
Volume169
Issue number1
StatePublished - Jul 1 2002

Fingerprint

Muromegalovirus
T-Lymphocytes
Genes
Genomic Library
Herpesviridae
Epitopes
Clone Cells
Infection
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

The murine cytomegalovirus immunomodulatory gene m152 prevents recognition of infected cells by M45-specific CTL but does not alter the immunodominance of the M45-specific CD8 T cell response in vivo. / Gold, Marielle; Munks, Michael; Wagner, Markus; Koszinowski, Ulrich H.; Hill, Ann; Fling, Steven P.

In: Journal of Immunology, Vol. 169, No. 1, 01.07.2002, p. 359-365.

Research output: Contribution to journalArticle

@article{aa7e087313ff4e018b0a4039f38330d1,
title = "The murine cytomegalovirus immunomodulatory gene m152 prevents recognition of infected cells by M45-specific CTL but does not alter the immunodominance of the M45-specific CD8 T cell response in vivo",
abstract = "Although in vitro studies have shown that herpesviruses, including murine CMV (MCMV), encode genes that interfere with the MHC class I pathway, their effects on the CTL response in vivo is unclear. We identified a Db-restricted CTL epitope from MCMV M45 by screening an MCMV genomic library using CTL clones isolated from mice infected with MCMV lacking m152. Because m152 severely inhibits CTL recognition of M45 in vitro, we questioned whether an M45-specific response would be generated in mice infected with wild-type MCMV expressing m152. Mice infected with wild-type MCMV or MCMVΔm152 made similar responses to the M45 Ag. Moreover, we saw no skewing of the proportion of M45-specific CD8 T cells within the total MCMV-specific response after infection with MCMV with m152. Despite the profound effect m152 has on presentation of M45 in vitro, it does not affect the immunodominance of M45 in the CTL response in vivo.",
author = "Marielle Gold and Michael Munks and Markus Wagner and Koszinowski, {Ulrich H.} and Ann Hill and Fling, {Steven P.}",
year = "2002",
month = "7",
day = "1",
language = "English (US)",
volume = "169",
pages = "359--365",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "1",

}

TY - JOUR

T1 - The murine cytomegalovirus immunomodulatory gene m152 prevents recognition of infected cells by M45-specific CTL but does not alter the immunodominance of the M45-specific CD8 T cell response in vivo

AU - Gold, Marielle

AU - Munks, Michael

AU - Wagner, Markus

AU - Koszinowski, Ulrich H.

AU - Hill, Ann

AU - Fling, Steven P.

PY - 2002/7/1

Y1 - 2002/7/1

N2 - Although in vitro studies have shown that herpesviruses, including murine CMV (MCMV), encode genes that interfere with the MHC class I pathway, their effects on the CTL response in vivo is unclear. We identified a Db-restricted CTL epitope from MCMV M45 by screening an MCMV genomic library using CTL clones isolated from mice infected with MCMV lacking m152. Because m152 severely inhibits CTL recognition of M45 in vitro, we questioned whether an M45-specific response would be generated in mice infected with wild-type MCMV expressing m152. Mice infected with wild-type MCMV or MCMVΔm152 made similar responses to the M45 Ag. Moreover, we saw no skewing of the proportion of M45-specific CD8 T cells within the total MCMV-specific response after infection with MCMV with m152. Despite the profound effect m152 has on presentation of M45 in vitro, it does not affect the immunodominance of M45 in the CTL response in vivo.

AB - Although in vitro studies have shown that herpesviruses, including murine CMV (MCMV), encode genes that interfere with the MHC class I pathway, their effects on the CTL response in vivo is unclear. We identified a Db-restricted CTL epitope from MCMV M45 by screening an MCMV genomic library using CTL clones isolated from mice infected with MCMV lacking m152. Because m152 severely inhibits CTL recognition of M45 in vitro, we questioned whether an M45-specific response would be generated in mice infected with wild-type MCMV expressing m152. Mice infected with wild-type MCMV or MCMVΔm152 made similar responses to the M45 Ag. Moreover, we saw no skewing of the proportion of M45-specific CD8 T cells within the total MCMV-specific response after infection with MCMV with m152. Despite the profound effect m152 has on presentation of M45 in vitro, it does not affect the immunodominance of M45 in the CTL response in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0036644562&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036644562&partnerID=8YFLogxK

M3 - Article

C2 - 12077265

AN - SCOPUS:0036644562

VL - 169

SP - 359

EP - 365

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 1

ER -