The multi-adaptor proto-oncoprotein Cbl is a key regulator of Rac and actin assembly

Robin M. Scaife, Sara A. Courtneidge, Wallace Y. Langdon

Research output: Contribution to journalReview article

30 Scopus citations

Abstract

The induction of protein tyrosine kinase signaling pathways is a principal mechanism for promoting cellular activation. Biochemical and genetic analyses have implicated the multi-adaptor proto-oncogene protein Cbl as a key negative regulator of activated protein tyrosine kinases. By inhibiting the function of Cbl as a multi-domain adaptor protein, through expression of a truncated form (480-Cbl), we demonstrate that Cbl is a potent negative regulator of actin assembly in response to receptor tyrosine kinase (RTK) activation. Expression of 480-Cbl dramatically enhances RTK-dependent induction of actin dorsal ruffles, which correlates with a pronounced increase in Rac activation. By contrast, mitogenic signaling by RTK targets, such as PI 3-kinase and MAP kinases, as well as RTK-mediated tyrosine phosphorylation do not appear to be affected by 480-Cbl expression. Further, we determined that Cbl undergoes a striking RTK-activation-dependent translocation to sites of active actin dorsal ruffle nucleation. Hence, the selective regulation of RTK signaling to the actin cytoskeleton appears to result from recruitment of signaling proteins on a Cbl template bound to the actin cytoskeleton.

Original languageEnglish (US)
Pages (from-to)463-473
Number of pages11
JournalJournal of Cell Science
Volume116
Issue number3
DOIs
StatePublished - Feb 1 2003

Keywords

  • Actin
  • Cbl
  • RTK
  • Rac
  • Src

ASJC Scopus subject areas

  • Cell Biology

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