The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression

Paraic A. Kenny, Genee Y. Lee, Connie A. Myers, Richard M. Neve, Jeremy R. Semeiks, Paul T. Spellman, Katrin Lorenz, Eva H. Lee, Mary Helen Barcellos-Hoff, Ole W. Petersen, Joe W. Gray, Mina J. Bissell

Research output: Contribution to journalArticle

621 Scopus citations

Abstract

3D cell cultures are rapidly becoming the method of choice for the physiologically relevant modeling of many aspects of non-malignant and malignant cell behavior ex vivo. Nevertheless, only a limited number of distinct cell types have been evaluated in this assay to date. Here we report the first large scale comparison of the transcriptional profiles and 3D cell culture phenotypes of a substantial panel of human breast cancer cell lines. Each cell line adopts a colony morphology of one of four main classes in 3D culture. These morphologies reflect, at least in part, the underlying gene expression profile and protein expression patterns of the cell lines, and distinct morphologies were also associated with tumor cell invasiveness and with cell lines originating from metastases. We further demonstrate that consistent differences in genes encoding signal transduction proteins emerge when even tumor cells are cultured in 3D microenvironments.

Original languageEnglish (US)
Pages (from-to)84-96
Number of pages13
JournalMolecular Oncology
Volume1
Issue number1
DOIs
StatePublished - Jun 2007

Keywords

  • 3D culture
  • Breast cancer
  • Extracellular matrix
  • Gene expression profiling
  • Signal transduction

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Oncology
  • Cancer Research

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  • Cite this

    Kenny, P. A., Lee, G. Y., Myers, C. A., Neve, R. M., Semeiks, J. R., Spellman, P. T., Lorenz, K., Lee, E. H., Barcellos-Hoff, M. H., Petersen, O. W., Gray, J. W., & Bissell, M. J. (2007). The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression. Molecular Oncology, 1(1), 84-96. https://doi.org/10.1016/j.molonc.2007.02.004