The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression

Paraic A. Kenny, Genee Y. Lee, Connie A. Myers, Richard M. Neve, Jeremy R. Semeiks, Paul Spellman, Katrin Lorenz, Eva H. Lee, Mary Helen Barcellos-Hoff, Ole W. Petersen, Joe Gray, Mina J. Bissell

Research output: Contribution to journalArticle

584 Citations (Scopus)

Abstract

3D cell cultures are rapidly becoming the method of choice for the physiologically relevant modeling of many aspects of non-malignant and malignant cell behavior ex vivo. Nevertheless, only a limited number of distinct cell types have been evaluated in this assay to date. Here we report the first large scale comparison of the transcriptional profiles and 3D cell culture phenotypes of a substantial panel of human breast cancer cell lines. Each cell line adopts a colony morphology of one of four main classes in 3D culture. These morphologies reflect, at least in part, the underlying gene expression profile and protein expression patterns of the cell lines, and distinct morphologies were also associated with tumor cell invasiveness and with cell lines originating from metastases. We further demonstrate that consistent differences in genes encoding signal transduction proteins emerge when even tumor cells are cultured in 3D microenvironments.

Original languageEnglish (US)
Pages (from-to)84-96
Number of pages13
JournalMolecular Oncology
Volume1
Issue number1
DOIs
StatePublished - Jun 2007
Externally publishedYes

Fingerprint

Transcriptome
Breast Neoplasms
Cell Line
Cell Culture Techniques
Cultured Tumor Cells
Signal Transduction
Proteins
Cell Count
Neoplasm Metastasis
Phenotype
Genes
Neoplasms

Keywords

  • 3D culture
  • Breast cancer
  • Extracellular matrix
  • Gene expression profiling
  • Signal transduction

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Medicine

Cite this

The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression. / Kenny, Paraic A.; Lee, Genee Y.; Myers, Connie A.; Neve, Richard M.; Semeiks, Jeremy R.; Spellman, Paul; Lorenz, Katrin; Lee, Eva H.; Barcellos-Hoff, Mary Helen; Petersen, Ole W.; Gray, Joe; Bissell, Mina J.

In: Molecular Oncology, Vol. 1, No. 1, 06.2007, p. 84-96.

Research output: Contribution to journalArticle

Kenny, PA, Lee, GY, Myers, CA, Neve, RM, Semeiks, JR, Spellman, P, Lorenz, K, Lee, EH, Barcellos-Hoff, MH, Petersen, OW, Gray, J & Bissell, MJ 2007, 'The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression', Molecular Oncology, vol. 1, no. 1, pp. 84-96. https://doi.org/10.1016/j.molonc.2007.02.004
Kenny, Paraic A. ; Lee, Genee Y. ; Myers, Connie A. ; Neve, Richard M. ; Semeiks, Jeremy R. ; Spellman, Paul ; Lorenz, Katrin ; Lee, Eva H. ; Barcellos-Hoff, Mary Helen ; Petersen, Ole W. ; Gray, Joe ; Bissell, Mina J. / The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression. In: Molecular Oncology. 2007 ; Vol. 1, No. 1. pp. 84-96.
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