The modulation of platelet adhesion and activation by chitosan through plasma and extracellular matrix proteins

Megan S. Lord, Bill Cheng, Simon McCarthy, MoonSun Jung, John M. Whitelock

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Chitosan has been shown to promote initial wound closure events to prevent blood loss. Platelet adhesion and activation are crucial early events in these processes after traumatic bleeding leading to thrombus formation. Platelet adhesion to chitosan was found to be enhanced in the presence of adsorbed plasma and extracellular matrix proteins and was found to be primarily mediated by α IIbβ 3 integrins, while α 2β 1 integrins were found to be involved in platelet adhesion to collagen and perlecan. Platelets were found to be activated by chitosan, as shown by an increase in the expression of α IIbβ 3 integrins and P-selectin, while the extent of activation was modulated by the presence of proteins including perlecan and fibrinogen. Collagen-coated chitosan was found to activate platelets to the same extent as either chitosan or collagen alone. These data support the role of plasma and extracellular matrix proteins in promoting chitosan mediated platelet adhesion and activation supporting the hypothesis that chitosan promotes wound healing via these interactions.

Original languageEnglish (US)
Pages (from-to)6655-6662
Number of pages8
JournalBiomaterials
Volume32
Issue number28
DOIs
StatePublished - Oct 2011
Externally publishedYes

Fingerprint

Extracellular Matrix Proteins
Platelet Activation
Chitosan
Platelets
Adhesion
Chemical activation
Modulation
Proteins
Plasmas
Blood Platelets
Collagen
Integrins
P-Selectin
Wound Healing
Fibrinogen
Thrombosis
Blood
Hemorrhage
Wounds and Injuries

Keywords

  • Chitosan
  • Integrins
  • Platelets
  • Protein adsorption

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

The modulation of platelet adhesion and activation by chitosan through plasma and extracellular matrix proteins. / Lord, Megan S.; Cheng, Bill; McCarthy, Simon; Jung, MoonSun; Whitelock, John M.

In: Biomaterials, Vol. 32, No. 28, 10.2011, p. 6655-6662.

Research output: Contribution to journalArticle

Lord, Megan S. ; Cheng, Bill ; McCarthy, Simon ; Jung, MoonSun ; Whitelock, John M. / The modulation of platelet adhesion and activation by chitosan through plasma and extracellular matrix proteins. In: Biomaterials. 2011 ; Vol. 32, No. 28. pp. 6655-6662.
@article{0506edf85d604f2b8bd4fd99258abf54,
title = "The modulation of platelet adhesion and activation by chitosan through plasma and extracellular matrix proteins",
abstract = "Chitosan has been shown to promote initial wound closure events to prevent blood loss. Platelet adhesion and activation are crucial early events in these processes after traumatic bleeding leading to thrombus formation. Platelet adhesion to chitosan was found to be enhanced in the presence of adsorbed plasma and extracellular matrix proteins and was found to be primarily mediated by α IIbβ 3 integrins, while α 2β 1 integrins were found to be involved in platelet adhesion to collagen and perlecan. Platelets were found to be activated by chitosan, as shown by an increase in the expression of α IIbβ 3 integrins and P-selectin, while the extent of activation was modulated by the presence of proteins including perlecan and fibrinogen. Collagen-coated chitosan was found to activate platelets to the same extent as either chitosan or collagen alone. These data support the role of plasma and extracellular matrix proteins in promoting chitosan mediated platelet adhesion and activation supporting the hypothesis that chitosan promotes wound healing via these interactions.",
keywords = "Chitosan, Integrins, Platelets, Protein adsorption",
author = "Lord, {Megan S.} and Bill Cheng and Simon McCarthy and MoonSun Jung and Whitelock, {John M.}",
year = "2011",
month = "10",
doi = "10.1016/j.biomaterials.2011.05.062",
language = "English (US)",
volume = "32",
pages = "6655--6662",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "28",

}

TY - JOUR

T1 - The modulation of platelet adhesion and activation by chitosan through plasma and extracellular matrix proteins

AU - Lord, Megan S.

AU - Cheng, Bill

AU - McCarthy, Simon

AU - Jung, MoonSun

AU - Whitelock, John M.

PY - 2011/10

Y1 - 2011/10

N2 - Chitosan has been shown to promote initial wound closure events to prevent blood loss. Platelet adhesion and activation are crucial early events in these processes after traumatic bleeding leading to thrombus formation. Platelet adhesion to chitosan was found to be enhanced in the presence of adsorbed plasma and extracellular matrix proteins and was found to be primarily mediated by α IIbβ 3 integrins, while α 2β 1 integrins were found to be involved in platelet adhesion to collagen and perlecan. Platelets were found to be activated by chitosan, as shown by an increase in the expression of α IIbβ 3 integrins and P-selectin, while the extent of activation was modulated by the presence of proteins including perlecan and fibrinogen. Collagen-coated chitosan was found to activate platelets to the same extent as either chitosan or collagen alone. These data support the role of plasma and extracellular matrix proteins in promoting chitosan mediated platelet adhesion and activation supporting the hypothesis that chitosan promotes wound healing via these interactions.

AB - Chitosan has been shown to promote initial wound closure events to prevent blood loss. Platelet adhesion and activation are crucial early events in these processes after traumatic bleeding leading to thrombus formation. Platelet adhesion to chitosan was found to be enhanced in the presence of adsorbed plasma and extracellular matrix proteins and was found to be primarily mediated by α IIbβ 3 integrins, while α 2β 1 integrins were found to be involved in platelet adhesion to collagen and perlecan. Platelets were found to be activated by chitosan, as shown by an increase in the expression of α IIbβ 3 integrins and P-selectin, while the extent of activation was modulated by the presence of proteins including perlecan and fibrinogen. Collagen-coated chitosan was found to activate platelets to the same extent as either chitosan or collagen alone. These data support the role of plasma and extracellular matrix proteins in promoting chitosan mediated platelet adhesion and activation supporting the hypothesis that chitosan promotes wound healing via these interactions.

KW - Chitosan

KW - Integrins

KW - Platelets

KW - Protein adsorption

UR - http://www.scopus.com/inward/record.url?scp=79960564826&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960564826&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2011.05.062

DO - 10.1016/j.biomaterials.2011.05.062

M3 - Article

VL - 32

SP - 6655

EP - 6662

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 28

ER -