The mitochondrial ABC transporter Atm1p functions as a homodimer

Maja Chloupková, Scott K. Reaves, Linda M. LeBard, David Koeller

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The ATP-binding cassette (ABC) transporters constitute one of the largest families of proteins in evolution. The ATM1 gene of the yeast Saccharomyces cerevisiae encodes an ABC protein, which is localized to the mitochondrial inner membrane. A deletion of ATM1 results in the accumulation of up to a 30-fold excess of mitochondrial iron, loss of mitochondrial cytochromes and abnormalities of cytosolic iron metabolism. In this study, we have evaluated the role of conserved sequence elements in Atm1p in its function and dimerization in vivo. We report that conserved residues in the Walker A and B motifs of the nucleotide binding domain, which are required for ATP binding and hydrolysis, are essential for Atm1p function. In addition, we provide evidence that ATP binding is important for Atm1p dimerization.

Original languageEnglish (US)
Pages (from-to)65-69
Number of pages5
JournalFEBS Letters
Volume569
Issue number1-3
DOIs
StatePublished - Jul 2 2004

Fingerprint

ATP-Binding Cassette Transporters
Dimerization
Adenosine Triphosphate
Yeast
Iron
Nucleotide Motifs
Conserved Sequence
Mitochondrial Membranes
Cytochromes
Metabolism
Saccharomyces cerevisiae
Hydrolysis
Carrier Proteins
Proteins
Nucleotides
Genes
Yeasts
Membranes

Keywords

  • ABC transporter
  • ABC, ATP-binding cassette
  • CFTR, cystic fibrosis transmembrane conductance regulator
  • HC, Hartwell's complete medium
  • IP, immunoprecipitation
  • Iron metabolism
  • Mitochondrion
  • NBD, nucleotide binding domain
  • TMD, transmembrane domain

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The mitochondrial ABC transporter Atm1p functions as a homodimer. / Chloupková, Maja; Reaves, Scott K.; LeBard, Linda M.; Koeller, David.

In: FEBS Letters, Vol. 569, No. 1-3, 02.07.2004, p. 65-69.

Research output: Contribution to journalArticle

Chloupková, Maja ; Reaves, Scott K. ; LeBard, Linda M. ; Koeller, David. / The mitochondrial ABC transporter Atm1p functions as a homodimer. In: FEBS Letters. 2004 ; Vol. 569, No. 1-3. pp. 65-69.
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