The min K channel underlies the cardiac potassium current I(Ks) and mediates species-specific responses to protein kinase C

M. D. Varnum, A. E. Busch, C. T. Bond, J. Maylie, J. P. Adelman

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

A clone encoding the guinea pig (gp) min K potassium channel was isolated and expressed in Xenopus oocytes. The currents, gpI(sK), exhibit many of the electrophysiological and pharmacological properties characteristic of gpI(Ks), the slow component of the delayed rectifier potassium conductance in guinea pig cardiac myocytes. Depolarizing commands evoke outward potassium currents that activate slowly, with time constants on the order of seconds. The currents are blocked by the class III antiarrhythmic compound clofilium but not by the sotalol derivative E4031 or low concentrations of lanthanum. Like I(Ks) in guinea pig myocytes, gpI(sK) is modulated by stimulation of protein kinase A and protein kinase C (PKC). In contrast to rat and mouse I(sK), which are decreased upon stimulation of PKC, myocyte I(K) and gpI(sK) in oocytes are increased after PKC stimulation. Substitution of an asparagine residue at position 102 by serine (N102S), the residue found in the analogous position of the mouse and rat min K proteins, results in decreased gpI(sK) in response to PKC stimulation. These results support the hypothesis that the min K protein underlies the slow component of the delayed rectifier potassium current in ventricular myocytes and account for the species-specific responses to stimulation of PKC.

Original languageEnglish (US)
Pages (from-to)11528-11532
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number24
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • General

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