The microRNA miR-124 antagonizes the anti-neural REST/SCP1 pathway during embryonic CNS development

Jaya Visvanathan, Seunghee Lee, Bora Lee, Jae W. Lee, Soo Kyung Lee

Research output: Contribution to journalArticle

516 Scopus citations

Abstract

Neuronal gene expression is tightly regulated in developing CNS. Here, we demonstrate the anti-neural function of phosphatase SCP1 (small C-terminal domain phosphatase 1) during development. We further show that the neuron-enriched microRNA miR-124 directly targets SCP1-3′ untranslated region (UTR) to suppress SCP1 expression. In developing spinal cord, expression of miR-124 and SCP1 is complementary, and miR-124 antagonism phenocopies SCP1 overexpression and vice versa. In P19 cells, miR-124 suppresses SCP1 expression and induces neurogenesis, and SCP1 counteracts this proneural activity of miR-124. Our results suggest that, during CNS development, timely down-regulation of SCP1 is critical for inducing neurogenesis, and miR-124 contributes to this process at least in part by down-regulating SCP1 expression.

Original languageEnglish (US)
Pages (from-to)744-749
Number of pages6
JournalGenes and Development
Volume21
Issue number7
DOIs
StatePublished - Apr 1 2007

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Keywords

  • Neural tube development
  • Neurogenesis
  • SCP1
  • miR-124

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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