The Metalloproteinase Stromelysin-1 (Transin) Mediates PC12 Cell Growth Cone Invasiveness through Basal Laminae

Lora A. Nordstrom, Janis Lochner, Wendy Yeung, Gary Ciment

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Matrix metalloproteinases have been implicated in various extracellular matrix remodeling events that occur during normal development and in a number of pathologies. In previous work with PC12 rat pheochromocytoma cells, we found that the matrix metalloproteinase stromelysin-1 (ST1) was highly induced by nerve growth factor (NGF), but not by epidermal growth factor (EGF). Here, we show that ST1 immunoreactivity is present in growth cones of NGF-treated PC12 calls, but not EGF-treated or untreated cells. To determine whether ST1 expression confers neurite invasiveness, three lines of PC12 cells were produced that constitutively express ST1 antisense mRNA. These lines expressed and secreted significantly reduced levels of ST1 protein, as determined by immunoblot and immunocytochemical methods, but otherwise responded normally to NGF-treatment by elaborating neurites. We found, however, that the neurites of these ST1 antisense cells showed a significantly reduced ability to penetrate a Matrigel reconstituted basal lamina, as compared to the parental cells, suggesting that ST1 confers neurite invasiveness. Finally, we show that ST1 is also expressed in vivo in sections through Embryonic Day 15 rat embryos, including neurons of both the peripheral and central nervous systems. These date indicate that ST1 may play a role in axonal growth in vivo, including a role in growth cone invasiveness.

Original languageEnglish (US)
Pages (from-to)56-68
Number of pages13
JournalMolecular and Cellular Neuroscience
Volume6
Issue number1
DOIs
StatePublished - Feb 1995

Fingerprint

Matrix Metalloproteinase 3
Growth Cones
PC12 Cells
Metalloproteases
Basement Membrane
Neurites
Nerve Growth Factor
Epidermal Growth Factor
Matrix Metalloproteinase 1
Peripheral Nervous System
Pheochromocytoma
Matrix Metalloproteinases
Extracellular Matrix
Embryonic Structures
Central Nervous System
Pathology
Neurons
Messenger RNA

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Molecular Biology
  • Cell Biology
  • Developmental Neuroscience

Cite this

The Metalloproteinase Stromelysin-1 (Transin) Mediates PC12 Cell Growth Cone Invasiveness through Basal Laminae. / Nordstrom, Lora A.; Lochner, Janis; Yeung, Wendy; Ciment, Gary.

In: Molecular and Cellular Neuroscience, Vol. 6, No. 1, 02.1995, p. 56-68.

Research output: Contribution to journalArticle

Nordstrom, Lora A. ; Lochner, Janis ; Yeung, Wendy ; Ciment, Gary. / The Metalloproteinase Stromelysin-1 (Transin) Mediates PC12 Cell Growth Cone Invasiveness through Basal Laminae. In: Molecular and Cellular Neuroscience. 1995 ; Vol. 6, No. 1. pp. 56-68.
@article{ca7fb1aa499f4f59829f09d48422fb90,
title = "The Metalloproteinase Stromelysin-1 (Transin) Mediates PC12 Cell Growth Cone Invasiveness through Basal Laminae",
abstract = "Matrix metalloproteinases have been implicated in various extracellular matrix remodeling events that occur during normal development and in a number of pathologies. In previous work with PC12 rat pheochromocytoma cells, we found that the matrix metalloproteinase stromelysin-1 (ST1) was highly induced by nerve growth factor (NGF), but not by epidermal growth factor (EGF). Here, we show that ST1 immunoreactivity is present in growth cones of NGF-treated PC12 calls, but not EGF-treated or untreated cells. To determine whether ST1 expression confers neurite invasiveness, three lines of PC12 cells were produced that constitutively express ST1 antisense mRNA. These lines expressed and secreted significantly reduced levels of ST1 protein, as determined by immunoblot and immunocytochemical methods, but otherwise responded normally to NGF-treatment by elaborating neurites. We found, however, that the neurites of these ST1 antisense cells showed a significantly reduced ability to penetrate a Matrigel reconstituted basal lamina, as compared to the parental cells, suggesting that ST1 confers neurite invasiveness. Finally, we show that ST1 is also expressed in vivo in sections through Embryonic Day 15 rat embryos, including neurons of both the peripheral and central nervous systems. These date indicate that ST1 may play a role in axonal growth in vivo, including a role in growth cone invasiveness.",
author = "Nordstrom, {Lora A.} and Janis Lochner and Wendy Yeung and Gary Ciment",
year = "1995",
month = "2",
doi = "10.1006/mcne.1995.1006",
language = "English (US)",
volume = "6",
pages = "56--68",
journal = "Molecular and Cellular Neuroscience",
issn = "1044-7431",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - The Metalloproteinase Stromelysin-1 (Transin) Mediates PC12 Cell Growth Cone Invasiveness through Basal Laminae

AU - Nordstrom, Lora A.

AU - Lochner, Janis

AU - Yeung, Wendy

AU - Ciment, Gary

PY - 1995/2

Y1 - 1995/2

N2 - Matrix metalloproteinases have been implicated in various extracellular matrix remodeling events that occur during normal development and in a number of pathologies. In previous work with PC12 rat pheochromocytoma cells, we found that the matrix metalloproteinase stromelysin-1 (ST1) was highly induced by nerve growth factor (NGF), but not by epidermal growth factor (EGF). Here, we show that ST1 immunoreactivity is present in growth cones of NGF-treated PC12 calls, but not EGF-treated or untreated cells. To determine whether ST1 expression confers neurite invasiveness, three lines of PC12 cells were produced that constitutively express ST1 antisense mRNA. These lines expressed and secreted significantly reduced levels of ST1 protein, as determined by immunoblot and immunocytochemical methods, but otherwise responded normally to NGF-treatment by elaborating neurites. We found, however, that the neurites of these ST1 antisense cells showed a significantly reduced ability to penetrate a Matrigel reconstituted basal lamina, as compared to the parental cells, suggesting that ST1 confers neurite invasiveness. Finally, we show that ST1 is also expressed in vivo in sections through Embryonic Day 15 rat embryos, including neurons of both the peripheral and central nervous systems. These date indicate that ST1 may play a role in axonal growth in vivo, including a role in growth cone invasiveness.

AB - Matrix metalloproteinases have been implicated in various extracellular matrix remodeling events that occur during normal development and in a number of pathologies. In previous work with PC12 rat pheochromocytoma cells, we found that the matrix metalloproteinase stromelysin-1 (ST1) was highly induced by nerve growth factor (NGF), but not by epidermal growth factor (EGF). Here, we show that ST1 immunoreactivity is present in growth cones of NGF-treated PC12 calls, but not EGF-treated or untreated cells. To determine whether ST1 expression confers neurite invasiveness, three lines of PC12 cells were produced that constitutively express ST1 antisense mRNA. These lines expressed and secreted significantly reduced levels of ST1 protein, as determined by immunoblot and immunocytochemical methods, but otherwise responded normally to NGF-treatment by elaborating neurites. We found, however, that the neurites of these ST1 antisense cells showed a significantly reduced ability to penetrate a Matrigel reconstituted basal lamina, as compared to the parental cells, suggesting that ST1 confers neurite invasiveness. Finally, we show that ST1 is also expressed in vivo in sections through Embryonic Day 15 rat embryos, including neurons of both the peripheral and central nervous systems. These date indicate that ST1 may play a role in axonal growth in vivo, including a role in growth cone invasiveness.

UR - http://www.scopus.com/inward/record.url?scp=0028913162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028913162&partnerID=8YFLogxK

U2 - 10.1006/mcne.1995.1006

DO - 10.1006/mcne.1995.1006

M3 - Article

VL - 6

SP - 56

EP - 68

JO - Molecular and Cellular Neuroscience

JF - Molecular and Cellular Neuroscience

SN - 1044-7431

IS - 1

ER -