The melting of pulmonary surfactant monolayers

Wenfei Yan, Samares C. Biswas, Ted G. Laderas, Stephen (Steve) Hall

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Monomolecular films of phospholipids in the liquid-expanded (LE) phase after supercompression to high surface pressures (π), well above the equilibrium surface pressure (πe) at which fluid films collapse from the interface to form a three-dimensional bulk phase, and in the tilted-condensed (TC) phase both replicate the resistance to collapse that is characteristic of alveolar films in the lungs. To provide the basis for determining which film is present in the alveolus, we measured the melting characteristics of monolayers containing TC dipalmitoyl phosphatidylcholine (DPPC), as well as supercompressed 1-palmitoyl-2-oleoyl phosphatidylcholine and calf lung surfactant extract (CLSE). Films generated by appropriate manipulations on a captive bubble were heated from ≤27° C to ≥60° C at different constant π above πe. DPPC showed the abrupt expansion expected for the TC-LE phase transition, followed by the contraction produced by collapse. Supercompressed CLSE showed no evidence of the TC-LE expansion, arguing that supercompression did not simply convert the mixed lipid film to TC DPPC. For both DPPC and CLSE, the melting point, taken as the temperature at which collapse began, increased at higher π, in contrast to 1-palmitoyl-2-oleoyl phosphatidylcholine, for which higher π produced collapse at lower temperatures. For π between 50 and 65 mN/m, DPPC melted at 48-55° C, well above the main transition for bilayers at 41° C. At each π, CLSE melted at temperatures >10° C lower. The distinct melting points for TC DPPC and supercompressed CLSE provide the basis by which the nature of the alveolar film might be determined from the temperature-dependence of pulmonary mechanics.

Original languageEnglish (US)
Pages (from-to)1739-1745
Number of pages7
JournalJournal of Applied Physiology
Volume102
Issue number5
DOIs
StatePublished - May 2007

Fingerprint

Pulmonary Surfactants
1,2-Dipalmitoylphosphatidylcholine
Freezing
Surface-Active Agents
Lung
Temperature
Pressure
Phase Transition
Mechanics
Phospholipids
Lipids

Keywords

  • Captive bubble
  • Dipalmitoyl phosphatidylcholine
  • Jammed
  • Monolayer
  • Pulmonary mechanics
  • Supercompressed

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

The melting of pulmonary surfactant monolayers. / Yan, Wenfei; Biswas, Samares C.; Laderas, Ted G.; Hall, Stephen (Steve).

In: Journal of Applied Physiology, Vol. 102, No. 5, 05.2007, p. 1739-1745.

Research output: Contribution to journalArticle

Yan, Wenfei ; Biswas, Samares C. ; Laderas, Ted G. ; Hall, Stephen (Steve). / The melting of pulmonary surfactant monolayers. In: Journal of Applied Physiology. 2007 ; Vol. 102, No. 5. pp. 1739-1745.
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