The mechanism of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor desensitization after removal of glutamate

I. M. Raman, L. O. Trussell

Research output: Contribution to journalArticle

99 Scopus citations

Abstract

We have examined responses of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate) receptors in the chick nucleus magnocellularis to pairs of pulses of glutamate and determined the extent of desensitization and the rate of recovery. Receptors recovered from desensitization with a time constant of 16 ms, regardless of the concentration or duration of the conditioning pulse. Even with very brief conditioning pulses, evoking submaximal currents, desensitization occurred at a consistent rate after the removal of free ligand. A quantitative kinetic model based on these data shows that receptors must desensitize from a closed state. The results provide evidence that very brief exposure to glutamate, on the time scale of uniquantal synaptic transmission, will result in a significant reduction in sensitivity of postsynaptic receptors.

Original languageEnglish (US)
Pages (from-to)137-146
Number of pages10
JournalBiophysical Journal
Volume68
Issue number1
DOIs
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • Biophysics

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