The measurement of neuropeptide Y in discrete hypothalamic and limbic regions of male rhesus macaques with a human NPY-directed antiserum

O. Khorram; C. E. Roselli; W. E. Ellinwood; H. G. Spies

doi:10.1016/0196-9781(87)90180-X

The measurement of neuropeptide Y in discrete hypothalamic and limbic regions of male rhesus macaques with a human NPY-directed antiserum

O. Khorram, C. E. Roselli, W. E. Ellinwood, H. G. Spies

Chemical Physiology and Biochemistry

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The distribution of neuropeptide Y (NPY) in microdissected brain regions of male macaques was quantified with a specific radioimmunoassay (RIA). The RIA consisted of a specific antiserum (R31) against human NPY that could detect 7 pg/tube with an IC50 of 125 pg/tube at a final dilution of 1:20,000. Varying amounts of rabbit and monkey mediobasal hypothalami yielded parallel [¹²⁵I]NPY displacement curves in the assay and similar chromatographic elution profiles with those of synthetic human NPY. The NPY activity in acid extracts of discrete brain regions in castrate and castrated-testosterone-treated rhesus males was highest in mediobasal hypothalamus, followed by more rostral hypothalamic regions and amygdaloid nuclei. Testosterone did not alter NPY levels in any of the brain areas that we examined.

Original language	English (US)
Pages (from-to)	159-163
Number of pages	5
Journal	Peptides
Volume	8
Issue number	1
DOIs	https://doi.org/10.1016/0196-9781(87)90180-X
State	Published - 1987

Keywords

Brain
Microdissection
Neuropeptide Y
Radioimmunoassay
Testosterone

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/0196-9781(87)90180-X

Cite this

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title = "The measurement of neuropeptide Y in discrete hypothalamic and limbic regions of male rhesus macaques with a human NPY-directed antiserum",

abstract = "The distribution of neuropeptide Y (NPY) in microdissected brain regions of male macaques was quantified with a specific radioimmunoassay (RIA). The RIA consisted of a specific antiserum (R31) against human NPY that could detect 7 pg/tube with an IC50 of 125 pg/tube at a final dilution of 1:20,000. Varying amounts of rabbit and monkey mediobasal hypothalami yielded parallel [125I]NPY displacement curves in the assay and similar chromatographic elution profiles with those of synthetic human NPY. The NPY activity in acid extracts of discrete brain regions in castrate and castrated-testosterone-treated rhesus males was highest in mediobasal hypothalamus, followed by more rostral hypothalamic regions and amygdaloid nuclei. Testosterone did not alter NPY levels in any of the brain areas that we examined.",

keywords = "Brain, Microdissection, Neuropeptide Y, Radioimmunoassay, Testosterone",

author = "O. Khorram and Roselli, {C. E.} and Ellinwood, {W. E.} and Spies, {H. G.}",

note = "Funding Information: The 10 adult rhesus males (Macaca mulatta, 7-12 kg) in this study were caged individually in temperature (20--23°C) and light-controlled rooms (lights on 0600-1800 hr). They 1Publication No. 1503 of the Oregon Regional Primate Research Center; supported by National Institutes of Health Grants RR-00163, HD-16631, HD-18185 (to H.G.S.) and HD-16022 (to Dr. John A. Resko, OHSU). 2Special Fellow, Oregon Regional Primate Research Center, 1986. Present address: Texas Tech School of Medicine, Lubbock, TX. 3Requests for reprints should be addressed to Harold G. Spies, Ph.D., Associate Director for Research, Oregon Regional Primate Research Center, 505 N.W. 185th Avenue, Beaverton, OR 97006.",

year = "1987",

doi = "10.1016/0196-9781(87)90180-X",

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AU - Roselli, C. E.

AU - Ellinwood, W. E.

AU - Spies, H. G.

N1 - Funding Information: The 10 adult rhesus males (Macaca mulatta, 7-12 kg) in this study were caged individually in temperature (20--23°C) and light-controlled rooms (lights on 0600-1800 hr). They 1Publication No. 1503 of the Oregon Regional Primate Research Center; supported by National Institutes of Health Grants RR-00163, HD-16631, HD-18185 (to H.G.S.) and HD-16022 (to Dr. John A. Resko, OHSU). 2Special Fellow, Oregon Regional Primate Research Center, 1986. Present address: Texas Tech School of Medicine, Lubbock, TX. 3Requests for reprints should be addressed to Harold G. Spies, Ph.D., Associate Director for Research, Oregon Regional Primate Research Center, 505 N.W. 185th Avenue, Beaverton, OR 97006.

PY - 1987

Y1 - 1987

N2 - The distribution of neuropeptide Y (NPY) in microdissected brain regions of male macaques was quantified with a specific radioimmunoassay (RIA). The RIA consisted of a specific antiserum (R31) against human NPY that could detect 7 pg/tube with an IC50 of 125 pg/tube at a final dilution of 1:20,000. Varying amounts of rabbit and monkey mediobasal hypothalami yielded parallel [125I]NPY displacement curves in the assay and similar chromatographic elution profiles with those of synthetic human NPY. The NPY activity in acid extracts of discrete brain regions in castrate and castrated-testosterone-treated rhesus males was highest in mediobasal hypothalamus, followed by more rostral hypothalamic regions and amygdaloid nuclei. Testosterone did not alter NPY levels in any of the brain areas that we examined.

AB - The distribution of neuropeptide Y (NPY) in microdissected brain regions of male macaques was quantified with a specific radioimmunoassay (RIA). The RIA consisted of a specific antiserum (R31) against human NPY that could detect 7 pg/tube with an IC50 of 125 pg/tube at a final dilution of 1:20,000. Varying amounts of rabbit and monkey mediobasal hypothalami yielded parallel [125I]NPY displacement curves in the assay and similar chromatographic elution profiles with those of synthetic human NPY. The NPY activity in acid extracts of discrete brain regions in castrate and castrated-testosterone-treated rhesus males was highest in mediobasal hypothalamus, followed by more rostral hypothalamic regions and amygdaloid nuclei. Testosterone did not alter NPY levels in any of the brain areas that we examined.

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The measurement of neuropeptide Y in discrete hypothalamic and limbic regions of male rhesus macaques with a human NPY-directed antiserum

Abstract

Keywords

ASJC Scopus subject areas

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