The MarR-type repressor MhqR (YkvE) regulates multiple dioxygenases/ glyoxalases and an azoreductase which confer resistance to 2-methylhydroquinone and catechol in Bacillus subtilis

Stefanie Töwe, Montira Leelakriangsak, Kazuo Kobayashi, Nguyen Van Duy, Michael Hecker, Peter Zuber, Haike Antelmann

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Abstract

Catechol and 2-methylhydroquinone (2-MHQ) cause the induction of the thiol-specific stress response and four dioxygenases/glyoxalases in Bacillus subtilis. Using transcription factor arrays, the MarR-type regulator YkvE was identified as a repressor of the dioxygenase/glyoxalase-encoding mhqE gene. Transcriptional and proteome analyses of the ΔykvE mutant revealed the upregulation of ykcA (mhqA), ydfNOP (mhqNOP), yodED (mhqED) and yvaB (azoR2) encoding multiple dioxygenases/glyoxalases, oxidoreductases and an azoreductase. Primer extension experiments identified σA-type promoter sequences upstream of mhqA, mhqNOP, mhqED and azoR2 from which transcription is elevated after thiol stress. DNase I footprinting analysis showed that YkvE protects a primary imperfect inverted repeat with the consensus sequence of tATCTcgaAtTCgAGATaaaa in the azoR2, mhqE and mhqN promoter regions. Analysis of mhqE-promoter-bgaB fusions confirmed the significance of YkvE binding to this operator in vivo. Adjacent secondary repeats were protected by YkvE in the azoR2 and mhqN promoter regions consistent with multiple DNA-protein binding complexes. DNA-binding activity of YkvE was not directly affected by thiol-reactive compounds in vitro. Mutational analyses showed that MhqA, MhqO and AzoR2 confer resistance to 2-MHQ. Moreover, the ΔykvE mutant displayed a 2-MHQ and catechol resistant phenotype. YkvE was renamed as MhqR controlling a 2-MHQ and catechol-resistance regulon of B. subtilis.

Original languageEnglish (US)
Pages (from-to)40-54
Number of pages15
JournalMolecular Microbiology
Volume66
Issue number1
DOIs
StatePublished - Oct 2007

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Dioxygenases
Bacillus subtilis
Sulfhydryl Compounds
Genetic Promoter Regions
Regulon
Deoxyribonuclease I
Consensus Sequence
DNA-Binding Proteins
Proteome
Oxidoreductases
Transcription Factors
Up-Regulation
Phenotype
azoreductase
catechol
2-methyl-1,4-hydroquinone
DNA
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

The MarR-type repressor MhqR (YkvE) regulates multiple dioxygenases/ glyoxalases and an azoreductase which confer resistance to 2-methylhydroquinone and catechol in Bacillus subtilis. / Töwe, Stefanie; Leelakriangsak, Montira; Kobayashi, Kazuo; Van Duy, Nguyen; Hecker, Michael; Zuber, Peter; Antelmann, Haike.

In: Molecular Microbiology, Vol. 66, No. 1, 10.2007, p. 40-54.

Research output: Contribution to journalArticle

Töwe, Stefanie ; Leelakriangsak, Montira ; Kobayashi, Kazuo ; Van Duy, Nguyen ; Hecker, Michael ; Zuber, Peter ; Antelmann, Haike. / The MarR-type repressor MhqR (YkvE) regulates multiple dioxygenases/ glyoxalases and an azoreductase which confer resistance to 2-methylhydroquinone and catechol in Bacillus subtilis. In: Molecular Microbiology. 2007 ; Vol. 66, No. 1. pp. 40-54.
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abstract = "Catechol and 2-methylhydroquinone (2-MHQ) cause the induction of the thiol-specific stress response and four dioxygenases/glyoxalases in Bacillus subtilis. Using transcription factor arrays, the MarR-type regulator YkvE was identified as a repressor of the dioxygenase/glyoxalase-encoding mhqE gene. Transcriptional and proteome analyses of the ΔykvE mutant revealed the upregulation of ykcA (mhqA), ydfNOP (mhqNOP), yodED (mhqED) and yvaB (azoR2) encoding multiple dioxygenases/glyoxalases, oxidoreductases and an azoreductase. Primer extension experiments identified σA-type promoter sequences upstream of mhqA, mhqNOP, mhqED and azoR2 from which transcription is elevated after thiol stress. DNase I footprinting analysis showed that YkvE protects a primary imperfect inverted repeat with the consensus sequence of tATCTcgaAtTCgAGATaaaa in the azoR2, mhqE and mhqN promoter regions. Analysis of mhqE-promoter-bgaB fusions confirmed the significance of YkvE binding to this operator in vivo. Adjacent secondary repeats were protected by YkvE in the azoR2 and mhqN promoter regions consistent with multiple DNA-protein binding complexes. DNA-binding activity of YkvE was not directly affected by thiol-reactive compounds in vitro. Mutational analyses showed that MhqA, MhqO and AzoR2 confer resistance to 2-MHQ. Moreover, the ΔykvE mutant displayed a 2-MHQ and catechol resistant phenotype. YkvE was renamed as MhqR controlling a 2-MHQ and catechol-resistance regulon of B. subtilis.",
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T1 - The MarR-type repressor MhqR (YkvE) regulates multiple dioxygenases/ glyoxalases and an azoreductase which confer resistance to 2-methylhydroquinone and catechol in Bacillus subtilis

AU - Töwe, Stefanie

AU - Leelakriangsak, Montira

AU - Kobayashi, Kazuo

AU - Van Duy, Nguyen

AU - Hecker, Michael

AU - Zuber, Peter

AU - Antelmann, Haike

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