The lpf fimbrial operon mediates adhesion of Salmonella typhimurium to murine Peyer's patches

Andreas J. Bäumler, Renée M. Tsolis, Fred Heffron

    Research output: Contribution to journalArticle

    197 Citations (Scopus)

    Abstract

    We investigated the role of the Salmonella typhimurium fimbrial operon formed by the genes lpfABCDE in infection of mice. A mutant in lpfC, the gene encoding the fimbrial outer membrane usher, had an ≃5-fold increased 50% lethal dose when administered orally to mice. When mice were infected with a mixture of the lpfC mutant and isogenic wild-type S. typhimurium, the lpfC mutant was recovered in lower numbers from Peyer's patches, mesenteric lymph nodes, liver, and spleen. In an organ culture model using murine intestinal loops, lpfC mutants were shown to be associated in lower numbers than wild- type bacteria with Peyer's patches but not with villous intestine. The defect of the lpfC mutant in adhesion to Peyer's patches could be complemented by introducing lpfABCDE on a cosmid. Similarly, heterologous expression of the Salmonella lpf operon in Escherichia coli resulted in an increased adhesion to histological thin sections of Peyer's patch lymph follicles. Electron microscopic analysis of histological sections taken from Peyer's patches after intragastric infection of mice showed that, in contrast to the S. typhimurium wild type, the isogenic lpfC mutant did not destroy M cells of the follicle-associated epithelium. These data show that the Salmonella lpf operon is involved in adhesion to murine Peyer's patches.

    Original languageEnglish (US)
    Pages (from-to)279-283
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume93
    Issue number1
    DOIs
    StatePublished - Jan 9 1996

    Fingerprint

    Peyer's Patches
    Salmonella typhimurium
    Operon
    Salmonella
    Cosmids
    Organ Culture Techniques
    Lethal Dose 50
    Lymph
    Infection
    Genes
    Intestines
    Spleen
    Epithelium
    Lymph Nodes
    Electrons
    Escherichia coli
    Bacteria
    Membranes
    Liver

    Keywords

    • adhesin
    • fimbriae
    • M cells

    ASJC Scopus subject areas

    • Genetics
    • General

    Cite this

    The lpf fimbrial operon mediates adhesion of Salmonella typhimurium to murine Peyer's patches. / Bäumler, Andreas J.; Tsolis, Renée M.; Heffron, Fred.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 1, 09.01.1996, p. 279-283.

    Research output: Contribution to journalArticle

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