The local forces acting on the mechanotransduction channel in hair cell stereocilia

Richard J. Powers, Sue Kulason, Erdinc Atilgan, William E. Brownell, Sean X. Sun, Peter Barr-Gillespie, Alexander A. Spector

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In hair cells, mechanotransduction channels are located in the membrane of stereocilia tips, where the base of the tip link is attached. The tip-link force determines the system of other forces in the immediate channel environment, which change the channel open probability. This system of forces includes components that are out of plane and in plane relative to the membrane; the magnitude and direction of these components depend on the channel environment and arrangement. Using a computational model, we obtained the major forces involved as functions of the force applied via the tip link at the center of the membrane. We simulated factors related to channels and the membrane, including finite-sized channels located centrally or acentrally, stiffness of the hypothesized channel-cytoskeleton tether, and bending modulus of the membrane. Membrane forces are perpendicular to the directions of the principal curvatures of the deformed membrane. Our approach allows for a fine vectorial picture of the local forces gating the channel; membrane forces change with the membrane curvature and are themselves sufficient to affect the open probability of the channel.

Original languageEnglish (US)
Pages (from-to)2519-2528
Number of pages10
JournalBiophysical Journal
Volume106
Issue number11
DOIs
StatePublished - Jun 3 2014

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Stereocilia
Membranes
Cytoskeleton
Ion Channels

ASJC Scopus subject areas

  • Biophysics
  • Medicine(all)

Cite this

Powers, R. J., Kulason, S., Atilgan, E., Brownell, W. E., Sun, S. X., Barr-Gillespie, P., & Spector, A. A. (2014). The local forces acting on the mechanotransduction channel in hair cell stereocilia. Biophysical Journal, 106(11), 2519-2528. https://doi.org/10.1016/j.bpj.2014.03.034

The local forces acting on the mechanotransduction channel in hair cell stereocilia. / Powers, Richard J.; Kulason, Sue; Atilgan, Erdinc; Brownell, William E.; Sun, Sean X.; Barr-Gillespie, Peter; Spector, Alexander A.

In: Biophysical Journal, Vol. 106, No. 11, 03.06.2014, p. 2519-2528.

Research output: Contribution to journalArticle

Powers, RJ, Kulason, S, Atilgan, E, Brownell, WE, Sun, SX, Barr-Gillespie, P & Spector, AA 2014, 'The local forces acting on the mechanotransduction channel in hair cell stereocilia', Biophysical Journal, vol. 106, no. 11, pp. 2519-2528. https://doi.org/10.1016/j.bpj.2014.03.034
Powers, Richard J. ; Kulason, Sue ; Atilgan, Erdinc ; Brownell, William E. ; Sun, Sean X. ; Barr-Gillespie, Peter ; Spector, Alexander A. / The local forces acting on the mechanotransduction channel in hair cell stereocilia. In: Biophysical Journal. 2014 ; Vol. 106, No. 11. pp. 2519-2528.
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