The LLNL high‐speed sorter: Design features, operational characteristics, and biological utility

D. Peters, E. Branscomb, P. Dean, T. Merrill, D. Pinkel, M. Van Dilla, J. W. Gray

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

This report describes a dual‐beam, high‐speed sorter (HiSS) with a droplet production rate of 220,000 s−1 now in routine use at the Lawrence Livermore National Laboratory. The system can process and sort objects at rates in excess of 20,000 s−1. We report here on the development of HiSS, describe its operational characteristics, and evaluate its utility for analysis and purification of human chromosomes, human erythrocytes, and living Chinese hamster ovary cells.

Original languageEnglish (US)
Pages (from-to)290-301
Number of pages12
JournalCytometry
Volume6
Issue number4
DOIs
StatePublished - Jul 1985

Keywords

  • High‐speed sorting
  • cell sorting
  • chromosome sorting
  • dual‐beam flow cytometry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

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