The LLNL high‐speed sorter: Design features, operational characteristics, and biological utility

D. Peters; E. Branscomb; P. Dean; T. Merrill; D. Pinkel; M. Van Dilla; J. W. Gray

doi:10.1002/cyto.990060404

The LLNL high‐speed sorter: Design features, operational characteristics, and biological utility

D. Peters, E. Branscomb, P. Dean, T. Merrill, D. Pinkel, M. Van Dilla, J. W. Gray

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

This report describes a dual‐beam, high‐speed sorter (HiSS) with a droplet production rate of 220,000 s⁻¹ now in routine use at the Lawrence Livermore National Laboratory. The system can process and sort objects at rates in excess of 20,000 s⁻¹. We report here on the development of HiSS, describe its operational characteristics, and evaluate its utility for analysis and purification of human chromosomes, human erythrocytes, and living Chinese hamster ovary cells.

Original language	English (US)
Pages (from-to)	290-301
Number of pages	12
Journal	Cytometry
Volume	6
Issue number	4
DOIs	https://doi.org/10.1002/cyto.990060404
State	Published - Jul 1985
Externally published	Yes

Keywords

High‐speed sorting
cell sorting
chromosome sorting
dual‐beam flow cytometry

ASJC Scopus subject areas

Pathology and Forensic Medicine
Biophysics
Hematology
Endocrinology
Cell Biology

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10.1002/cyto.990060404

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AU - Pinkel, D.

AU - Van Dilla, M.

AU - Gray, J. W.

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AB - This report describes a dual‐beam, high‐speed sorter (HiSS) with a droplet production rate of 220,000 s−1 now in routine use at the Lawrence Livermore National Laboratory. The system can process and sort objects at rates in excess of 20,000 s−1. We report here on the development of HiSS, describe its operational characteristics, and evaluate its utility for analysis and purification of human chromosomes, human erythrocytes, and living Chinese hamster ovary cells.

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KW - chromosome sorting

KW - dual‐beam flow cytometry

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The LLNL high‐speed sorter: Design features, operational characteristics, and biological utility

Abstract

Keywords

ASJC Scopus subject areas

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