The Legionella IcmS-IcmW protein complex is important for Dot/Icm-mediated protein translocation

Shira Ninio, Deborah M. Zuckman-Cholon, Eric Cambronne, Craig R. Roy

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

The intracellular pathogen Legionella pneumophila can infect and replicate within macrophages of a human host. To establish infection, Legionella require the Dot/Icm secretion system to inject protein substrates directly into the host cell cytoplasm. The mechanism by which substrate proteins are engaged and translocated by the Dot/Icm system is not well understood. Here we show that two cytosolic components of the Dot/Icm secretion machinery, the proteins IcmS and EcmW, play an important role in substrate translocation. Biochemical analysis indicates that IcmS and IcmW form a stable protein complex. In Legionella, the IcmW protein is rapidly degraded in the absence of the IcmS protein. Substrate proteins translocated into mammalian host cells by the Dot/Icm system were identified using the IcmW protein as bait in a yeast two-hybrid screen. It was determined that the IcmS-IcmW complex interacts with these substrates and plays an important role in translocation of these proteins into mammalian cells. These data are consistent with the IcmS-IcmW complex being involved in the recognition and Dot/ Icm-dependent translocation of substrate proteins during Legionella infection of host cells.

Original languageEnglish (US)
Pages (from-to)912-926
Number of pages15
JournalMolecular Microbiology
Volume55
Issue number3
DOIs
StatePublished - Feb 2005
Externally publishedYes

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Legionella
Protein Transport
Proteins
Legionella pneumophila
Infection
Cytoplasm
Yeasts
Macrophages

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

The Legionella IcmS-IcmW protein complex is important for Dot/Icm-mediated protein translocation. / Ninio, Shira; Zuckman-Cholon, Deborah M.; Cambronne, Eric; Roy, Craig R.

In: Molecular Microbiology, Vol. 55, No. 3, 02.2005, p. 912-926.

Research output: Contribution to journalArticle

Ninio, Shira ; Zuckman-Cholon, Deborah M. ; Cambronne, Eric ; Roy, Craig R. / The Legionella IcmS-IcmW protein complex is important for Dot/Icm-mediated protein translocation. In: Molecular Microbiology. 2005 ; Vol. 55, No. 3. pp. 912-926.
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