The lack of cardiotrophin-1 alters expression of interleukin-6 and leukemia inhibitory factor mRNA but does not impair cardiac injury response

Kurt Gritman, Donna M. Van Winkle, Christina U. Lorentz, Diane Pennica, Beth Habecker

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Cardiotrophin-1 (CT-1) was identified as a growth factor for cardiac myocytes and CT-1 protects myocytes from cell death. Adult CT-1-/- mice exhibit neural deficits including the loss of preganglionic sympathetic neurons, but their autonomic and cardiac parameters have not been examined. We used these mice to determine if the absence of CT-1 or loss of preganglionic sympathetic input altered heart rate, left ventricular pressure, cardiac contractility (dP/dt), or cell death following ischemia-reperfusion. Basal heart rate was increased in CT-1-/- mice, and this difference was abolished by ganglionic block. Left ventricular pressure and dP/dt were unchanged. Dobutamine stimulated similar increases in heart rate and dP/dt in both genotypes, but ventricular pressure was significantly lower in CT-1 nulls. Cardiac expression of interleukin-6 (IL-6) mRNA was increased significantly in CT-1 null mice, while leukemia inhibitory factor (LIF) mRNA was unchanged. Infarct size normalized to area at risk was no different in CT-1-/- mice (33.8 ± 1.0% vs. 37.7 ± 3.2% WT) 24 h after ischemia-reperfusion. Induction of IL-6 mRNA after infarct was significantly abrogated in CT-1 null mice compared to wild-type mice, but LIF mRNA-induction remained significant in CT-1 null mice and might contribute to cardiac protection in the absence of CT-1.

Original languageEnglish (US)
Pages (from-to)9-16
Number of pages8
JournalCytokine
Volume36
Issue number1-2
DOIs
StatePublished - Oct 2006

Fingerprint

Leukemia Inhibitory Factor
Interleukin-6
Messenger RNA
Wounds and Injuries
Ventricular Pressure
Heart Rate
Cell death
Reperfusion
cardiotrophin 1
Cell Death
Ischemia
Dobutamine
Cardiac Myocytes
Muscle Cells
Neurons
Intercellular Signaling Peptides and Proteins
Genotype

Keywords

  • Cardiac protection
  • Cardiotrophin-1
  • gp130
  • Interleukin-6
  • Leukemia inhibitory factor

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

The lack of cardiotrophin-1 alters expression of interleukin-6 and leukemia inhibitory factor mRNA but does not impair cardiac injury response. / Gritman, Kurt; Van Winkle, Donna M.; Lorentz, Christina U.; Pennica, Diane; Habecker, Beth.

In: Cytokine, Vol. 36, No. 1-2, 10.2006, p. 9-16.

Research output: Contribution to journalArticle

Gritman, Kurt ; Van Winkle, Donna M. ; Lorentz, Christina U. ; Pennica, Diane ; Habecker, Beth. / The lack of cardiotrophin-1 alters expression of interleukin-6 and leukemia inhibitory factor mRNA but does not impair cardiac injury response. In: Cytokine. 2006 ; Vol. 36, No. 1-2. pp. 9-16.
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