The invasive and destructive behavior of HIV-induced T cell syncytia on collagen and endothelium

Andrew Sylwester, Karla Daniels, David R. Soll

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

HIV-induced syncytia of the CD4+ SUP-T1 T cell line mimic the subcellular organization of single cells and are able to crawl like single cells through extension of giant pseudopods. Because syncytia have been demonstrated in lymphoid tissue of HIV-positive individuals, their behavior has been investigated on more natural substrata, including dehydrated collagen, hydrated collagen, endothelial monolayers, and endothelial monolayers grown on collagen cushions. On hydrated collagen gels, both individual SUPT1 cells and syncytia form unusually long cylindrical projections that possess pseudopodial ends and are highly dynamic. Syncytia penetrate collagen gels through extension of these projections and disrupt their integrity. When incubated on endothelium, both single cells and syncytia readily traverse the monolayer through holes, and when incubated on endothelium supported by a collagen cushion, syncytia generate large holes through the monolayer, penetrate the monolayer, and disrupt the collagen gel through extension of long, complex projections. Invading syncytia also release viruses in a polarized fashion which adhere to and are taken up in vesicles by the endothelium. It is suggested that the destructive behaviors of syncytia which have been demonstrated in vitro may have correlates in vivo.

Original languageEnglish (US)
Pages (from-to)233-244
Number of pages12
JournalJournal of Leukocyte Biology
Volume63
Issue number2
StatePublished - Feb 1998
Externally publishedYes

Fingerprint

Giant Cells
Endothelium
Collagen
HIV
T-Lymphocytes
Gels
Virus Release
Pseudopodia
Lymphoid Tissue
Cell Line

Keywords

  • Pseudopods
  • SUP-T1

ASJC Scopus subject areas

  • Cell Biology

Cite this

The invasive and destructive behavior of HIV-induced T cell syncytia on collagen and endothelium. / Sylwester, Andrew; Daniels, Karla; Soll, David R.

In: Journal of Leukocyte Biology, Vol. 63, No. 2, 02.1998, p. 233-244.

Research output: Contribution to journalArticle

@article{cc15d990f48b4c05994e6931261209b4,
title = "The invasive and destructive behavior of HIV-induced T cell syncytia on collagen and endothelium",
abstract = "HIV-induced syncytia of the CD4+ SUP-T1 T cell line mimic the subcellular organization of single cells and are able to crawl like single cells through extension of giant pseudopods. Because syncytia have been demonstrated in lymphoid tissue of HIV-positive individuals, their behavior has been investigated on more natural substrata, including dehydrated collagen, hydrated collagen, endothelial monolayers, and endothelial monolayers grown on collagen cushions. On hydrated collagen gels, both individual SUPT1 cells and syncytia form unusually long cylindrical projections that possess pseudopodial ends and are highly dynamic. Syncytia penetrate collagen gels through extension of these projections and disrupt their integrity. When incubated on endothelium, both single cells and syncytia readily traverse the monolayer through holes, and when incubated on endothelium supported by a collagen cushion, syncytia generate large holes through the monolayer, penetrate the monolayer, and disrupt the collagen gel through extension of long, complex projections. Invading syncytia also release viruses in a polarized fashion which adhere to and are taken up in vesicles by the endothelium. It is suggested that the destructive behaviors of syncytia which have been demonstrated in vitro may have correlates in vivo.",
keywords = "Pseudopods, SUP-T1",
author = "Andrew Sylwester and Karla Daniels and Soll, {David R.}",
year = "1998",
month = "2",
language = "English (US)",
volume = "63",
pages = "233--244",
journal = "Journal of Leukocyte Biology",
issn = "0741-5400",
publisher = "FASEB",
number = "2",

}

TY - JOUR

T1 - The invasive and destructive behavior of HIV-induced T cell syncytia on collagen and endothelium

AU - Sylwester, Andrew

AU - Daniels, Karla

AU - Soll, David R.

PY - 1998/2

Y1 - 1998/2

N2 - HIV-induced syncytia of the CD4+ SUP-T1 T cell line mimic the subcellular organization of single cells and are able to crawl like single cells through extension of giant pseudopods. Because syncytia have been demonstrated in lymphoid tissue of HIV-positive individuals, their behavior has been investigated on more natural substrata, including dehydrated collagen, hydrated collagen, endothelial monolayers, and endothelial monolayers grown on collagen cushions. On hydrated collagen gels, both individual SUPT1 cells and syncytia form unusually long cylindrical projections that possess pseudopodial ends and are highly dynamic. Syncytia penetrate collagen gels through extension of these projections and disrupt their integrity. When incubated on endothelium, both single cells and syncytia readily traverse the monolayer through holes, and when incubated on endothelium supported by a collagen cushion, syncytia generate large holes through the monolayer, penetrate the monolayer, and disrupt the collagen gel through extension of long, complex projections. Invading syncytia also release viruses in a polarized fashion which adhere to and are taken up in vesicles by the endothelium. It is suggested that the destructive behaviors of syncytia which have been demonstrated in vitro may have correlates in vivo.

AB - HIV-induced syncytia of the CD4+ SUP-T1 T cell line mimic the subcellular organization of single cells and are able to crawl like single cells through extension of giant pseudopods. Because syncytia have been demonstrated in lymphoid tissue of HIV-positive individuals, their behavior has been investigated on more natural substrata, including dehydrated collagen, hydrated collagen, endothelial monolayers, and endothelial monolayers grown on collagen cushions. On hydrated collagen gels, both individual SUPT1 cells and syncytia form unusually long cylindrical projections that possess pseudopodial ends and are highly dynamic. Syncytia penetrate collagen gels through extension of these projections and disrupt their integrity. When incubated on endothelium, both single cells and syncytia readily traverse the monolayer through holes, and when incubated on endothelium supported by a collagen cushion, syncytia generate large holes through the monolayer, penetrate the monolayer, and disrupt the collagen gel through extension of long, complex projections. Invading syncytia also release viruses in a polarized fashion which adhere to and are taken up in vesicles by the endothelium. It is suggested that the destructive behaviors of syncytia which have been demonstrated in vitro may have correlates in vivo.

KW - Pseudopods

KW - SUP-T1

UR - http://www.scopus.com/inward/record.url?scp=0031882075&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031882075&partnerID=8YFLogxK

M3 - Article

C2 - 9468282

AN - SCOPUS:0031882075

VL - 63

SP - 233

EP - 244

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

IS - 2

ER -