The intrinsic structural asymmetry of highly curved phospholipid bilayer membranes

Adela Chrzeszczyk, Arnold Wishnia, Charles S. Springer

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

Phosphorus-31 NMR studies of solutions of small l-α-dipalmitoyl phosphatidylcholine bilayer vesicles containing sodium dimethyl phosphate uniformly distributed between the continuous external and the intravesicular aqueous spaces, with the paramagnetic shift reagent Pr3+ present only in the external space, are reported. These studies give the distribution both of dipalmitoyl phosphatidylcholine in the vesicle inner and outer monolayers and of dimethyl phosphate in the aqueous spaes. With the third necessary parameter obtained from the vesicle sedimentation coefficient, the very different packing parameters of dipalmitoyl phosphatidylcholine in inner and outer monolayers can be determined. The vesicle outer radius is 109 Å. Although the total bilayer thickness is virtually identical to that of planar bilayers, the outer monolayer is thicker (20 Å) and the inner monolayer thinner (15 Å). The area per head group at the inner surface, 68 Å2, is like the planar value, but the tails are much more folded, so as to decrease the radial lengths and increase the tangential spread (to 94 Å2). The reverse is true in the outer layer: the surface per head group is 76 Å2, tapering to 51 Å2 in the tail region, so that outer layer tails are relatively extended. The difference is equivalent to a shift of about two 2g1 kinks from outer to inner layers; the uneven packing certainly affects fluidity, and may have important biological consequences.

Original languageEnglish (US)
Pages (from-to)161-169
Number of pages9
JournalBBA - Biomembranes
Volume470
Issue number2
DOIs
StatePublished - Oct 17 1977

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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