The interaction of nitric oxide and superoxide in the human fetal- placental vasculature

G. Holcberg, W. Kossenjans, M. Miodovnik, Leslie Myatt

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

OBJECTIVE: Our purpose was to study the interaction of nitric oxide and superoxide anion on the vasculature of the isolated perfused human placental cotyledon. STUDY DESIGN: Isolated placental cotyledons were preconstricted with the thromboxane mimetic U46619, and fetal perfusion pressure was used as an index of response. The interaction of nitric oxide and O2 - was studied by three protocols: (1) with endogenous nitric oxide, (2) with addition of exogenous nitric oxide, and (3) with inhibition of nitric oxide synthesis by the nitric oxide synthase inhibitor N-nitro-L-arginine. O2 was generated by infusion of purine-xanthine oxidase. Statistical significance of response to treatment was determined by paired t test. RESULTS: Infusion of xanthine oxidase with purine in the presence of endogenous nitric oxide resulted in vasodilation (p <0.05). Vasodilation was more pronounced in the presence of exogenous nitric oxide (p <0.008). Coinfusion of xanthine oxidase with purine in the presence of N-nitro-L-arginine to inhibit nitric oxide synthesis resulted in vasoconstriction. CONCLUSION: We conclude that, rather than superoxide inactivating nitric oxide, interaction of the two radicals generates in the placental vasculature a vasodilator, which may be peroxynitrite.

Original languageEnglish (US)
Pages (from-to)528-533
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume173
Issue number2
StatePublished - 1995
Externally publishedYes

Fingerprint

Superoxides
Nitric Oxide
Xanthine Oxidase
Cotyledon
Vasodilation
Arginine
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Peroxynitrous Acid
Thromboxanes
Vasoconstriction
Vasodilator Agents
Nitric Oxide Synthase
Perfusion
Pressure

Keywords

  • Nitric oxide
  • peroxynitrite
  • placenta
  • purine
  • superoxide
  • xanthine oxidase

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

The interaction of nitric oxide and superoxide in the human fetal- placental vasculature. / Holcberg, G.; Kossenjans, W.; Miodovnik, M.; Myatt, Leslie.

In: American Journal of Obstetrics and Gynecology, Vol. 173, No. 2, 1995, p. 528-533.

Research output: Contribution to journalArticle

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AB - OBJECTIVE: Our purpose was to study the interaction of nitric oxide and superoxide anion on the vasculature of the isolated perfused human placental cotyledon. STUDY DESIGN: Isolated placental cotyledons were preconstricted with the thromboxane mimetic U46619, and fetal perfusion pressure was used as an index of response. The interaction of nitric oxide and O2 - was studied by three protocols: (1) with endogenous nitric oxide, (2) with addition of exogenous nitric oxide, and (3) with inhibition of nitric oxide synthesis by the nitric oxide synthase inhibitor N-nitro-L-arginine. O2 was generated by infusion of purine-xanthine oxidase. Statistical significance of response to treatment was determined by paired t test. RESULTS: Infusion of xanthine oxidase with purine in the presence of endogenous nitric oxide resulted in vasodilation (p <0.05). Vasodilation was more pronounced in the presence of exogenous nitric oxide (p <0.008). Coinfusion of xanthine oxidase with purine in the presence of N-nitro-L-arginine to inhibit nitric oxide synthesis resulted in vasoconstriction. CONCLUSION: We conclude that, rather than superoxide inactivating nitric oxide, interaction of the two radicals generates in the placental vasculature a vasodilator, which may be peroxynitrite.

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