The interaction of monomeric and complexed mouse monoclonal IgG with rat basophilic leukemia cells: A subset of IgG molecules can bind to RBL cell Fc receptors for IgG

T. J. Hall, Marvin Rittenberg

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Rat basophilic leukemia (RBL) cells were shown to bind mouse monoclonal (MC) IgE and certain mouse monomeric IgG1 and IgG2b monoclonal antibodies (MAb) by using a haptenated sheep red blood cell (SRBC) rosetting assay. Rosette formation was antibody concentration dependent with all three immunoglobulin isotypes, but at least 100 times more IgG than IgE was required to form a similar number of rosettes. It was shown by FACS analysis and rosette formation that a subset (8/23) of the IgG MC was able to bind to RBL cells as monomers. However, the majority 15/23 did not bind or bound weakly (

Original languageEnglish (US)
Pages (from-to)2331-2338
Number of pages8
JournalJournal of Immunology
Volume137
Issue number7
StatePublished - 1986

Fingerprint

Fc Receptors
Rosette Formation
Leukemia
Immunoglobulin G
Immunoglobulin E
Immunoglobulin Isotypes
Sheep
Erythrocytes
Monoclonal Antibodies
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

The interaction of monomeric and complexed mouse monoclonal IgG with rat basophilic leukemia cells : A subset of IgG molecules can bind to RBL cell Fc receptors for IgG. / Hall, T. J.; Rittenberg, Marvin.

In: Journal of Immunology, Vol. 137, No. 7, 1986, p. 2331-2338.

Research output: Contribution to journalArticle

@article{70d71b816a6c4db6ba1b3026cc96d861,
title = "The interaction of monomeric and complexed mouse monoclonal IgG with rat basophilic leukemia cells: A subset of IgG molecules can bind to RBL cell Fc receptors for IgG",
abstract = "Rat basophilic leukemia (RBL) cells were shown to bind mouse monoclonal (MC) IgE and certain mouse monomeric IgG1 and IgG2b monoclonal antibodies (MAb) by using a haptenated sheep red blood cell (SRBC) rosetting assay. Rosette formation was antibody concentration dependent with all three immunoglobulin isotypes, but at least 100 times more IgG than IgE was required to form a similar number of rosettes. It was shown by FACS analysis and rosette formation that a subset (8/23) of the IgG MC was able to bind to RBL cells as monomers. However, the majority 15/23 did not bind or bound weakly (",
author = "Hall, {T. J.} and Marvin Rittenberg",
year = "1986",
language = "English (US)",
volume = "137",
pages = "2331--2338",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "7",

}

TY - JOUR

T1 - The interaction of monomeric and complexed mouse monoclonal IgG with rat basophilic leukemia cells

T2 - A subset of IgG molecules can bind to RBL cell Fc receptors for IgG

AU - Hall, T. J.

AU - Rittenberg, Marvin

PY - 1986

Y1 - 1986

N2 - Rat basophilic leukemia (RBL) cells were shown to bind mouse monoclonal (MC) IgE and certain mouse monomeric IgG1 and IgG2b monoclonal antibodies (MAb) by using a haptenated sheep red blood cell (SRBC) rosetting assay. Rosette formation was antibody concentration dependent with all three immunoglobulin isotypes, but at least 100 times more IgG than IgE was required to form a similar number of rosettes. It was shown by FACS analysis and rosette formation that a subset (8/23) of the IgG MC was able to bind to RBL cells as monomers. However, the majority 15/23 did not bind or bound weakly (

AB - Rat basophilic leukemia (RBL) cells were shown to bind mouse monoclonal (MC) IgE and certain mouse monomeric IgG1 and IgG2b monoclonal antibodies (MAb) by using a haptenated sheep red blood cell (SRBC) rosetting assay. Rosette formation was antibody concentration dependent with all three immunoglobulin isotypes, but at least 100 times more IgG than IgE was required to form a similar number of rosettes. It was shown by FACS analysis and rosette formation that a subset (8/23) of the IgG MC was able to bind to RBL cells as monomers. However, the majority 15/23 did not bind or bound weakly (

UR - http://www.scopus.com/inward/record.url?scp=0022470047&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022470047&partnerID=8YFLogxK

M3 - Article

C2 - 2944953

AN - SCOPUS:0022470047

VL - 137

SP - 2331

EP - 2338

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 7

ER -