TY - JOUR
T1 - The Influence of Root Canal Sealer on Extended Intracanal Survival of Enterococcus faecalis With and Without Gelatinase Production Ability in Obturated Root Canals
AU - Sedgley, Christine
PY - 2007/5
Y1 - 2007/5
N2 - Enterococcus faecalis can survive for extended periods in obturated root canals. In this study, the hypotheses tested were that long-term survival of E. faecalis is dependent on (1) the type of endodontic sealer and (2) the capacity for microbial gelatinase activity, a potential "virulence" trait identified previously in clinical isolates. Root canals of extracted human canines (n = 95) were inoculated with either E. faecalis OG1RF or its gelatinase-negative mutant E. faecalis TX5128. After 48 hours of incubation at 37°C, canals were obturated with gutta-percha and either the epoxy-resin-based AH-Plus (Dentsply, De Trey, Konstanz, Germany), the silicone-based RoekoSeal (Coltène/Whaledent, Langenau, Germany), or zinc oxide eugenol-based Roth's sealer (Root Canal Cement Type 801; Roth International Ltd, Chicago, IL). Access cavities were sealed. After 8 months of incubation at 37°C, viable E. faecalis was recovered from more teeth sealed with RoekoSeal (95%) compared with AH-Plus (40%) (p = 0.0004, Fisher's exact test) and Roth's sealer (45%) (p = 0.0012, Fisher's exact test). In the RoekoSeal groups, viable counts of E. faecalis OG1RF were higher than E. faecalis TX5128 (p = 0.03, Mann-Whitney U test) suggesting that gelatinase activity plays a role in long-term survival of E. faecalis in obturated root canals.
AB - Enterococcus faecalis can survive for extended periods in obturated root canals. In this study, the hypotheses tested were that long-term survival of E. faecalis is dependent on (1) the type of endodontic sealer and (2) the capacity for microbial gelatinase activity, a potential "virulence" trait identified previously in clinical isolates. Root canals of extracted human canines (n = 95) were inoculated with either E. faecalis OG1RF or its gelatinase-negative mutant E. faecalis TX5128. After 48 hours of incubation at 37°C, canals were obturated with gutta-percha and either the epoxy-resin-based AH-Plus (Dentsply, De Trey, Konstanz, Germany), the silicone-based RoekoSeal (Coltène/Whaledent, Langenau, Germany), or zinc oxide eugenol-based Roth's sealer (Root Canal Cement Type 801; Roth International Ltd, Chicago, IL). Access cavities were sealed. After 8 months of incubation at 37°C, viable E. faecalis was recovered from more teeth sealed with RoekoSeal (95%) compared with AH-Plus (40%) (p = 0.0004, Fisher's exact test) and Roth's sealer (45%) (p = 0.0012, Fisher's exact test). In the RoekoSeal groups, viable counts of E. faecalis OG1RF were higher than E. faecalis TX5128 (p = 0.03, Mann-Whitney U test) suggesting that gelatinase activity plays a role in long-term survival of E. faecalis in obturated root canals.
KW - Enterococcus faecalis
KW - gelatinase
KW - root canal sealer
KW - root canals
KW - survival
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U2 - 10.1016/j.joen.2007.01.010
DO - 10.1016/j.joen.2007.01.010
M3 - Article
C2 - 17437872
AN - SCOPUS:34147172847
VL - 33
SP - 561
EP - 566
JO - Journal of Endodontics
JF - Journal of Endodontics
SN - 0099-2399
IS - 5
ER -