The Influence of Root Canal Sealer on Extended Intracanal Survival of Enterococcus faecalis With and Without Gelatinase Production Ability in Obturated Root Canals

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10 Scopus citations

Abstract

Enterococcus faecalis can survive for extended periods in obturated root canals. In this study, the hypotheses tested were that long-term survival of E. faecalis is dependent on (1) the type of endodontic sealer and (2) the capacity for microbial gelatinase activity, a potential "virulence" trait identified previously in clinical isolates. Root canals of extracted human canines (n = 95) were inoculated with either E. faecalis OG1RF or its gelatinase-negative mutant E. faecalis TX5128. After 48 hours of incubation at 37°C, canals were obturated with gutta-percha and either the epoxy-resin-based AH-Plus (Dentsply, De Trey, Konstanz, Germany), the silicone-based RoekoSeal (Coltène/Whaledent, Langenau, Germany), or zinc oxide eugenol-based Roth's sealer (Root Canal Cement Type 801; Roth International Ltd, Chicago, IL). Access cavities were sealed. After 8 months of incubation at 37°C, viable E. faecalis was recovered from more teeth sealed with RoekoSeal (95%) compared with AH-Plus (40%) (p = 0.0004, Fisher's exact test) and Roth's sealer (45%) (p = 0.0012, Fisher's exact test). In the RoekoSeal groups, viable counts of E. faecalis OG1RF were higher than E. faecalis TX5128 (p = 0.03, Mann-Whitney U test) suggesting that gelatinase activity plays a role in long-term survival of E. faecalis in obturated root canals.

Original languageEnglish (US)
Pages (from-to)561-566
Number of pages6
JournalJournal of endodontics
Volume33
Issue number5
DOIs
StatePublished - May 1 2007

Keywords

  • Enterococcus faecalis
  • gelatinase
  • root canal sealer
  • root canals
  • survival

ASJC Scopus subject areas

  • Dentistry(all)

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