The infectious molecular clone and pseudotyped virus models of human immunodeficiency virus type 1 exhibit significant differences in virion composition with only moderate differences in infectivity and inhibition sensitivity

Nicholas M. Provine, Wendy Blay Puryear, Xueling Wu, Julie Overbaugh, Nancy L. Haigwood

    Research output: Contribution to journalArticle

    21 Scopus citations

    Abstract

    Two frequently employed methods for generating well-characterized, genetically defined infectious human immunodeficiency virus type 1 in vitro include the use of infectious molecular clones (IMCs) and pseudoviruses (PVs) competent for single-round infection. We compared six matched pairs of IMCs and PVs. The relative amounts of Env incorporated and efficiency of cleavage differed substantially between the two systems. Altering the ratio of proviral genome and env expression plasmids can produce pseudovirions that are structurally more similar to the matched IMCs. Differences in Env incorporation and cleavage translated into moderate differences in assays infectivity and sensitivity to neutralizing antibodies and entry inhibitors.

    Original languageEnglish (US)
    Pages (from-to)9002-9007
    Number of pages6
    JournalJournal of virology
    Volume83
    Issue number17
    DOIs
    StatePublished - Sep 22 2009

    ASJC Scopus subject areas

    • Microbiology
    • Immunology
    • Insect Science
    • Virology

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