The immunological characterization of several human ribonucleases by using primary binding tests

Edward Neuwelt, M. Schmukler, M. S. Niziak, P. B. Jewett, C. C. Levy

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

RNAases (ribonucleases), purified from 4 human tissues, as well as bovine pancreatic RNAase (RNAase A), were studied by immunodiffusion methods and by two different primary binding tests. The enzymes fell into two groups immunologically, those purified from plasma and pancreas in one and those from spleen and liver in the other. No antigenic cross-reaction between the two groups was detected by any of the immunoassays used. There was a slight antigenic cross reaction between the human and bovine pancreatic RNAases. The liver and spleen RNAases were immunologically identical by all criteria used, whereas a small but consistent antigenic difference between the human plasma and human pancreas enzymes was detected. The significance of this difference between human plasma and pancreas RNAases is discussed in relation to similarities and differences in their properties.

Original languageEnglish (US)
Pages (from-to)419-426
Number of pages8
JournalBiochemical Journal
Volume163
Issue number3
StatePublished - 1977
Externally publishedYes

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Plasma (human)
Ribonucleases
Liver
Pancreas
Cross Reactions
Enzymes
Spleen
Tissue
Plasmas
Immunodiffusion
Immunoassay

ASJC Scopus subject areas

  • Biochemistry

Cite this

Neuwelt, E., Schmukler, M., Niziak, M. S., Jewett, P. B., & Levy, C. C. (1977). The immunological characterization of several human ribonucleases by using primary binding tests. Biochemical Journal, 163(3), 419-426.

The immunological characterization of several human ribonucleases by using primary binding tests. / Neuwelt, Edward; Schmukler, M.; Niziak, M. S.; Jewett, P. B.; Levy, C. C.

In: Biochemical Journal, Vol. 163, No. 3, 1977, p. 419-426.

Research output: Contribution to journalArticle

Neuwelt, E, Schmukler, M, Niziak, MS, Jewett, PB & Levy, CC 1977, 'The immunological characterization of several human ribonucleases by using primary binding tests', Biochemical Journal, vol. 163, no. 3, pp. 419-426.
Neuwelt, Edward ; Schmukler, M. ; Niziak, M. S. ; Jewett, P. B. ; Levy, C. C. / The immunological characterization of several human ribonucleases by using primary binding tests. In: Biochemical Journal. 1977 ; Vol. 163, No. 3. pp. 419-426.
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