The Human Phosphatidylinositol Phosphatase SAC1 Interacts with the Coatomer I Complex

Holger M. Rohde, Fei Ying Cheong, Gerlinde Konrad, Karin Paiha, Peter Mayinger, Guido Boehmelt

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Abstract

The Saccharomyces cerevisiae SAC1 gene encodes an integral membrane protein of the endoplasmic reticulum (ER) and the Golgi apparatus. Yeast SAC1 mutants display a wide array of phenotypes including inositol auxotrophy, cold sensitivity, secretory defects, disturbed ATP transport into the ER, or suppression of actin gene mutations. At present, it is not clear how these phenotypes relate to the finding that SAC1 displays polyphosphoinositide phosphatase activity. Moreover, it is still an open question whether SAC1 functions similarly in mammalian cells, since some phenotypes are yeast-specific. Potential protein interaction partners and, connected to that, possible regulatory circuits have not been described. Therefore, we have cloned human SAC1 (hSAC1), show that it behaves similar to ySac1p in terms of substrate specificity, demonstrate that the endogenous protein localizes to the ER and Golgi, and identify for the first time members of the coatomer I (COPI) complex as interaction partners of hSAC1. Mutation of a putative COPI interaction motif (KXKXX) at its C terminus abolishes interaction with COPI and causes accumulation of hSAC1 in the Golgi. In addition, we generated a catalytically inactive mutant, demonstrate that its lipid binding capacity is unaltered, and show that it accumulates in the Golgi, incapable of interacting with the COPI complex despite the presence of the KXKXX motif. These results open the possibility that the enzymatic function of hSAC1 provides a switch for accessibility of the COPI interaction motif.

Original languageEnglish (US)
Pages (from-to)52689-52699
Number of pages11
JournalJournal of Biological Chemistry
Volume278
Issue number52
DOIs
StatePublished - Dec 26 2003

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Coat Protein Complex I
Phosphatidylinositols
Phosphoric Monoester Hydrolases
Endoplasmic Reticulum
Yeast
Phenotype
Genes
Yeasts
Mutation
Golgi Apparatus
Inositol
Substrate Specificity
Saccharomyces cerevisiae
Actins
Membrane Proteins
Proteins
Adenosine Triphosphate
Cells
Switches
Lipids

ASJC Scopus subject areas

  • Biochemistry

Cite this

The Human Phosphatidylinositol Phosphatase SAC1 Interacts with the Coatomer I Complex. / Rohde, Holger M.; Cheong, Fei Ying; Konrad, Gerlinde; Paiha, Karin; Mayinger, Peter; Boehmelt, Guido.

In: Journal of Biological Chemistry, Vol. 278, No. 52, 26.12.2003, p. 52689-52699.

Research output: Contribution to journalArticle

Rohde, Holger M. ; Cheong, Fei Ying ; Konrad, Gerlinde ; Paiha, Karin ; Mayinger, Peter ; Boehmelt, Guido. / The Human Phosphatidylinositol Phosphatase SAC1 Interacts with the Coatomer I Complex. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 52. pp. 52689-52699.
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