The higher plant Arabidopsis thaliana encodes a functional CDC48 homologue which is highly expressed in dividing and expanding cells

Heidi Feiler, T. Desprez, V. Santoni, J. Kronenberger, M. Caboche, J. Traas

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

We have identified an Arabidopsis thaliana CDC48 gene which, unlike the putative mammalian homologue valosin-containing protein (VCP), functionally complements Saccharomyces cerevisiae cdc48 mutants. CDC48 is an essential gene in S. cerevisiae and genetic studies suggest a role in spindle pole body separation. Biochemical studies link VCP function to membrane trafficking and signal transduction. We have described the AtCDC48 expression pattern in a multicellular eukaryote; the zones of cell division, expansion and differentiation are physically separated in higher plants, thus allowing the analysis of in situ expression patterns with respect to the state of cell proliferation. AtCDC48 is highly expressed in the proliferating cells of the vegetative shoot, root, floral inflorescence and flowers, and in rapidly growing cells. AtCDC48 mRNA and the encoded protein are up-regulated in the developing microspores and ovules. AtCDC48 expression is down-regulated in most differentiated cell types. AtCDC48p was primarily localized to the nucleus and, during cytokinesis, to the phragmoplast, a site where membrane vesicles are targeted in the deposition of new cell wall materials. This study shows that the essential cell division function of CDC48 has been conserved by, at least, some multicellular eukaryotes and suggests that in higher plants, CDC48 functions in cell division and growth processes.

Original languageEnglish (US)
Pages (from-to)5626-5637
Number of pages12
JournalEMBO Journal
Volume14
Issue number22
StatePublished - 1995
Externally publishedYes

Fingerprint

Arabidopsis
Cell Division
Cells
Eukaryota
Saccharomyces cerevisiae
Cell Growth Processes
Spindle Pole Bodies
Yeast
Ovule
Inflorescence
Cytokinesis
Membranes
Genes
Essential Genes
Signal transduction
Cell Wall
Cell Differentiation
Signal Transduction
Cell proliferation
Cell Proliferation

Keywords

  • Arabidopsis thaliana
  • ATPase
  • CAD
  • CDC48
  • VCP

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

The higher plant Arabidopsis thaliana encodes a functional CDC48 homologue which is highly expressed in dividing and expanding cells. / Feiler, Heidi; Desprez, T.; Santoni, V.; Kronenberger, J.; Caboche, M.; Traas, J.

In: EMBO Journal, Vol. 14, No. 22, 1995, p. 5626-5637.

Research output: Contribution to journalArticle

Feiler, H, Desprez, T, Santoni, V, Kronenberger, J, Caboche, M & Traas, J 1995, 'The higher plant Arabidopsis thaliana encodes a functional CDC48 homologue which is highly expressed in dividing and expanding cells', EMBO Journal, vol. 14, no. 22, pp. 5626-5637.
Feiler, Heidi ; Desprez, T. ; Santoni, V. ; Kronenberger, J. ; Caboche, M. ; Traas, J. / The higher plant Arabidopsis thaliana encodes a functional CDC48 homologue which is highly expressed in dividing and expanding cells. In: EMBO Journal. 1995 ; Vol. 14, No. 22. pp. 5626-5637.
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