Abstract
We have identified an Arabidopsis thaliana CDC48 gene which, unlike the putative mammalian homologue valosin-containing protein (VCP), functionally complements Saccharomyces cerevisiae cdc48 mutants. CDC48 is an essential gene in S. cerevisiae and genetic studies suggest a role in spindle pole body separation. Biochemical studies link VCP function to membrane trafficking and signal transduction. We have described the AtCDC48 expression pattern in a multicellular eukaryote; the zones of cell division, expansion and differentiation are physically separated in higher plants, thus allowing the analysis of in situ expression patterns with respect to the state of cell proliferation. AtCDC48 is highly expressed in the proliferating cells of the vegetative shoot, root, floral inflorescence and flowers, and in rapidly growing cells. AtCDC48 mRNA and the encoded protein are up-regulated in the developing microspores and ovules. AtCDC48 expression is down-regulated in most differentiated cell types. AtCDC48p was primarily localized to the nucleus and, during cytokinesis, to the phragmoplast, a site where membrane vesicles are targeted in the deposition of new cell wall materials. This study shows that the essential cell division function of CDC48 has been conserved by, at least, some multicellular eukaryotes and suggests that in higher plants, CDC48 functions in cell division and growth processes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5626-5637 |
| Number of pages | 12 |
| Journal | EMBO Journal |
| Volume | 14 |
| Issue number | 22 |
| State | Published - 1995 |
| Externally published | Yes |
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Keywords
- Arabidopsis thaliana
- ATPase
- CAD
- CDC48
- VCP
ASJC Scopus subject areas
- Cell Biology
- Genetics
Cite this
The higher plant Arabidopsis thaliana encodes a functional CDC48 homologue which is highly expressed in dividing and expanding cells. / Feiler, Heidi; Desprez, T.; Santoni, V.; Kronenberger, J.; Caboche, M.; Traas, J.
In: EMBO Journal, Vol. 14, No. 22, 1995, p. 5626-5637.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The higher plant Arabidopsis thaliana encodes a functional CDC48 homologue which is highly expressed in dividing and expanding cells
AU - Feiler, Heidi
AU - Desprez, T.
AU - Santoni, V.
AU - Kronenberger, J.
AU - Caboche, M.
AU - Traas, J.
PY - 1995
Y1 - 1995
N2 - We have identified an Arabidopsis thaliana CDC48 gene which, unlike the putative mammalian homologue valosin-containing protein (VCP), functionally complements Saccharomyces cerevisiae cdc48 mutants. CDC48 is an essential gene in S. cerevisiae and genetic studies suggest a role in spindle pole body separation. Biochemical studies link VCP function to membrane trafficking and signal transduction. We have described the AtCDC48 expression pattern in a multicellular eukaryote; the zones of cell division, expansion and differentiation are physically separated in higher plants, thus allowing the analysis of in situ expression patterns with respect to the state of cell proliferation. AtCDC48 is highly expressed in the proliferating cells of the vegetative shoot, root, floral inflorescence and flowers, and in rapidly growing cells. AtCDC48 mRNA and the encoded protein are up-regulated in the developing microspores and ovules. AtCDC48 expression is down-regulated in most differentiated cell types. AtCDC48p was primarily localized to the nucleus and, during cytokinesis, to the phragmoplast, a site where membrane vesicles are targeted in the deposition of new cell wall materials. This study shows that the essential cell division function of CDC48 has been conserved by, at least, some multicellular eukaryotes and suggests that in higher plants, CDC48 functions in cell division and growth processes.
AB - We have identified an Arabidopsis thaliana CDC48 gene which, unlike the putative mammalian homologue valosin-containing protein (VCP), functionally complements Saccharomyces cerevisiae cdc48 mutants. CDC48 is an essential gene in S. cerevisiae and genetic studies suggest a role in spindle pole body separation. Biochemical studies link VCP function to membrane trafficking and signal transduction. We have described the AtCDC48 expression pattern in a multicellular eukaryote; the zones of cell division, expansion and differentiation are physically separated in higher plants, thus allowing the analysis of in situ expression patterns with respect to the state of cell proliferation. AtCDC48 is highly expressed in the proliferating cells of the vegetative shoot, root, floral inflorescence and flowers, and in rapidly growing cells. AtCDC48 mRNA and the encoded protein are up-regulated in the developing microspores and ovules. AtCDC48 expression is down-regulated in most differentiated cell types. AtCDC48p was primarily localized to the nucleus and, during cytokinesis, to the phragmoplast, a site where membrane vesicles are targeted in the deposition of new cell wall materials. This study shows that the essential cell division function of CDC48 has been conserved by, at least, some multicellular eukaryotes and suggests that in higher plants, CDC48 functions in cell division and growth processes.
KW - Arabidopsis thaliana
KW - ATPase
KW - CAD
KW - CDC48
KW - VCP
UR - http://www.scopus.com/inward/record.url?scp=0028805251&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028805251&partnerID=8YFLogxK
M3 - Article
C2 - 8521820
AN - SCOPUS:0028805251
VL - 14
SP - 5626
EP - 5637
JO - EMBO Journal
JF - EMBO Journal
SN - 0261-4189
IS - 22
ER -